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Gene Review:

CVXgORF2 - ORF2

Cactus virus X

Davies, C. et al., Cruz, S.S. et al., Lin, N.S. et al., Angell, S.M. et al.

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Disease relevance of CVXgORF2

A modified form of PVX, PVX.GFP-CP which expressed a chimeric gene encoding a fusion between the 27-kDa Aequorea victoria green fluorescent protein and the amino terminus of the 25-kDa PVX coat protein, assembled into virions and moved both locally and systemically [1].
Sub-cellular localization of the 25-kDa protein encoded in the triple gene block of potato virus X [2].
Therefore, although mutational studies indicate a role for the 25-kDa protein in the cell to cell movement of viral infection it is unlikely to act in precisely the same manner as the other well-characterized movement proteins of tobacco mosaic and cowpea mosaic viruses [2].

High impact information on CVXgORF2

A mutant PVX with a deletion in the gene for the 25-kDa protein accumulated in inoculated protoplasts to the same level as the wild-type PVX [3].
In an in vitro rabbit reticulocyte lysate system, sBaMV RNA directed the synthesis of a protein estimated to be 25 kDa by SDS-polyacrylamide gel electrophoresis [4].
Immunogold labeling of thin sections of infected tissue revealed that the 25-kDa protein is associated with the cytoplasmic inclusion bodies characteristic of PVX infections, but not with the cell wall or plasmodesmata [2].
The 25-kDa protein of potato virus X (PVX) is encoded by the 5' open reading frame of the overlapping triple gene block, has a GKS sequence motif characteristic of nucleotide binding proteins, and is thought to play a role in movement between cells [2].

References

Assembly and movement of a plant virus carrying a green fluorescent protein overcoat. Cruz, S.S., Chapman, S., Roberts, A.G., Roberts, I.M., Prior, D.A., Oparka, K.J. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
Sub-cellular localization of the 25-kDa protein encoded in the triple gene block of potato virus X. Davies, C., Hills, G., Baulcombe, D.C. Virology (1993) [Pubmed]
Cell-to-cell movement of potato virus X is associated with a change in the size-exclusion limit of plasmodesmata in trichome cells of Nicotiana clevelandii. Angell, S.M., Davies, C., Baulcombe, D.C. Virology (1996) [Pubmed]
A satellite RNA associated with bamboo mosaic potexvirus. Lin, N.S., Hsu, Y.H. Virology (1994) [Pubmed]

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