Disease relevance of cdtA

• We report transcriptional analysis of cdt genes (cdtA, B, and C ) encoding cytolethal distending toxin (CDT) in Campylobacter jejuni and demonstrate that a luxS mutation affects the cdt transcription in C. jejuni [1].

High impact information on cdtA

• The first type of mutation comprised a 667-bp deletion across cdtA and cdtB and considerable degeneration in the remainder of the cdt locus [2].
• CDT activity requires the function of three genes: cdtA, cdtB, and cdtC [3].
• Promoter analysis of cytolethal distending toxin genes (cdtA , B, and C ) and effect of a luxS mutation on CDT production in Campylobacter jejuni [1].
• The reverse transcriptase-polymerase chain reaction (RT-PCR) showed that cdtA, cdtB, and cdtC genes constitute a polycistronic operon in C. jejuni [1].

Biological context of cdtA

• The nucleotide sequence of the genes revealed that there are three genes, cdtA, cdtB, and cdtC, encoding proteins with predicted sizes of 30,11-6, 28,989, and 21,157 Da, respectively [4].
• One hundred three (88%) isolates were positive for cdt gene cluster PCR detection (cdt gene cluster-PCR), whereas 101 (86.3%), 102 (87.2%), and 110 (94%) isolates were positive for cdtA-, cdtB-, and cdtC-PCR, respectively [5].

Other interactions of cdtA

• The toxin production was determined on the phenotypic level using a CHO-K1 cell culture model and on the genotypic level using PCR for detection of the cdtA, cdtB and cdtC genes [6].

References