High impact information on cup

• However, 43S complex recruitment remains inhibited in the absence of functional Cup, uncovering a second Bruno-dependent silencing mechanism [1].
• Validating current models, we directly demonstrate the mechanism proposed for Cup-mediated repression: inhibition of small ribosomal subunit recruitment to oskar mRNA [1].
• Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression [2].
• Thus, Smaug represses translation via a Cup-dependent block in eIF4G recruitment [2].
• Furthermore, eukaryotic initiation factor 4E (eIF4E) is localized within the oocyte in a cup-dependent manner and binds directly to Cup in vitro [3].

Biological context of cup

• In this report, we show that Nos interacts with Cup, which is required for normal development of the ovarian germline cells. nos and cup also interact genetically--reducing the level of cup activity specifically suppresses the oogenesis defects associated with the nos(RC) allele [4].
• The Drosophila gene fs(2)cup interacts with otu to define a cytoplasmic pathway required for the structure and function of germ-line chromosomes [5].

Anatomical context of cup

• In Drosophila, the product of the fs (2)cup gene (Cup) is known to be crucial for diverse aspects of female germ-line development [6].
• Surprisingly, biochemical purification of Tral revealed that it is part of a large RNA-protein complex that includes the translation/localization factors Me31B and Cup as well as the mRNAs for endoplasmic reticulum (ER) exit site components [7].

Regulatory relationships of cup

• A mutant Cup protein lacking this sequence fails to repress osk translation in vivo [8].

Other interactions of cup

• Here, we report that an ovarian protein, Cup, is required to repress precocious osk translation [8].
• We have analyzed a gene, fs(2)cup, that participates in many of the same processes and interacts with otu genetically [5].

References