The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

otu  -  ovarian tumor

Drosophila melanogaster

Synonyms: CG12743, DROOTUA, Dmel\CG12743, OTU, Otu, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of otu


High impact information on otu

  • It has been proposed that otu phenotypes result from abnormal germ cell division and differentiation [2].
  • To understand better what role otu performs in oogenesis we have undertaken an analysis of protein expression from the otu locus [2].
  • Immunostaining of otu proteins is restricted to the cytoplasm of germ cells, and a rapid loss of oocyte immunostaining during stage 11 suggests that there is a rapid and selective degradation of otu proteins within the oocyte but not within its 15 interconnected nurse cells [2].
  • Sequence analysis of otu cDNAs suggests that these proteins are translated from two mRNAs generated by alternative splicing of a 126-bp exon between the sixth and seventh exon of the smaller transcript [2].
  • An apparent null mutation causes abnormal cysts to form containing an excess number of cells that cannot differentiate into gametes. bam function resides within a simple 2.2-kb transcription unit encoding a single 442-amino-acid protein that shows similarity to the product of the ovarian tumor gene [3].

Biological context of otu


Anatomical context of otu

  • The possible role of otu in the determination of the sexual identity of germ cells has not been extensively explored [4].
  • Synergistic interaction between ovoD1 and otu alleles leads to the occasional transformation of chromosomally female germ cells into early spermatocytes [4].
  • We show that the transport is ineffective in the qui and otu mutants apparently due to the lack of a properly differentiated oocyte [7].
  • In salivary glands of SuUR and in the nurse cells of otu mutants, under-replication is partly suppressed and a banded structure appears within the centric heterochromatin of chromosome 3 [8].
  • Immunolocalization experiments demonstrated that otu protein is present in the cytoplasm of oogonial stem cells that populate third instar larvae and in all germ-line-derived cells until late in oogenesis [9].

Associations of otu with chemical compounds


Physical interactions of otu

  • Interestingly, the strongest OVO-binding site is very near the otu transcription start, where basal transcriptional complexes must function [6].
  • The Drosophila gene fs(2)cup interacts with otu to define a cytoplasmic pathway required for the structure and function of germ-line chromosomes [11].

Regulatory relationships of otu

  • The position of the otu locus in the regulatory cascade of germ line sex determination has been studied by using mutations that constitutively express the feminizing activity of the Sxl gene [4].
  • Drosophila OVO regulates ovarian tumor transcription by binding unusually near the transcription start site [6].
  • In particular, hfp mutants display striking defects in the developmentally regulated splicing of ovarian tumor (otu) [12].
  • Even more extreme shortening of life spans were observed when the sex peptide gene (Acp70A) was expressed in homozygous otu females, though they were virgin, indicating that the shortening in life span is due to seminal factors [13].

Other interactions of otu

  • Our analyses support dual cooperative roles for Sqd, Hrb27C and Otu during Drosophila oogenesis [5].
  • This shows that otu acts upstream of Sxl in the process of germ line sex determination [4].
  • Furthermore, transgenic expression of the missing otu splice form can rescue the ovarian phenotypes of hfp [12].
  • By testing for expression of male germline traits, we determined that partial germline sex transformation occurs in otu, snf, Sxlfs, and bam ovarian tumors [14].
  • Mutations in otu and ovo cause a range of ovarian defects, including agametic ovaries and tumorous egg cysts, but do not affect spermatogenesis [15].

Analytical, diagnostic and therapeutic context of otu

  • Reciprocal cross and developmental Northern blot studies suggest a maternal requirement for otu in the development of the female germline [16].


  1. Developmental analysis of the ovarian tumor gene during Drosophila oogenesis. Rodesch, C., Geyer, P.K., Patton, J.S., Bae, E., Nagoshi, R.N. Genetics (1995) [Pubmed]
  2. A specific ovarian tumor protein isoform is required for efficient differentiation of germ cells in Drosophila oogenesis. Steinhauer, W.R., Kalfayan, L.J. Genes Dev. (1992) [Pubmed]
  3. bag-of-marbles: a Drosophila gene required to initiate both male and female gametogenesis. McKearin, D.M., Spradling, A.C. Genes Dev. (1990) [Pubmed]
  4. The role of the ovarian tumor locus in Drosophila melanogaster germ line sex determination. Pauli, D., Oliver, B., Mahowald, A.P. Development (1993) [Pubmed]
  5. Hrb27C, Sqd and Otu cooperatively regulate gurken RNA localization and mediate nurse cell chromosome dispersion in Drosophila oogenesis. Goodrich, J.S., Clouse, K.N., Schüpbach, T. Development (2004) [Pubmed]
  6. Drosophila OVO regulates ovarian tumor transcription by binding unusually near the transcription start site. Lü, J., Oliver, B. Development (2001) [Pubmed]
  7. Analyses of the Drosophila quit, ovarian tumor and shut down mutants in oocyte differentiation using in situ hybridisation. Tirronen, M., Partanen, M., Heino, T.O., Heino, T.I., Roos, C. Mech. Dev. (1993) [Pubmed]
  8. Abnormal tissue-dependent polytenization of a block of chromosome 3 pericentric heterochromatin in Drosophila melanogaster. Koryakov, D.E., Domanitskaya, E.V., Belyakin, S.N., Zhimulev, I.F. J. Cell. Sci. (2003) [Pubmed]
  9. The ovarian tumor protein isoforms of Drosophila melanogaster exhibit differences in function, expression, and localization. Sass, G.L., Comer, A.R., Searles, L.L. Dev. Biol. (1995) [Pubmed]
  10. Sequence and structure of the Drosophila melanogaster ovarian tumor gene and generation of an antibody specific for the ovarian tumor protein. Steinhauer, W.R., Walsh, R.C., Kalfayan, L.J. Mol. Cell. Biol. (1989) [Pubmed]
  11. The Drosophila gene fs(2)cup interacts with otu to define a cytoplasmic pathway required for the structure and function of germ-line chromosomes. Keyes, L.N., Spradling, A.C. Development (1997) [Pubmed]
  12. Half pint regulates alternative splice site selection in Drosophila. Van Buskirk, C., Schüpbach, T. Dev. Cell (2002) [Pubmed]
  13. Enhanced cost of mating in female sterile mutants of Drosophila melanogaster. Ueyama, M., Fuyama, Y. Genes Genet. Syst. (2003) [Pubmed]
  14. Evidence for sex transformation of germline cells in ovarian tumor mutants of Drosophila. Wei, G., Oliver, B., Pauli, D., Mahowald, A.P. Dev. Biol. (1994) [Pubmed]
  15. The somatic sex determines the requirement for ovarian tumor gene activity in the proliferation of the Drosophila germline. Nagoshi, R.N., Patton, J.S., Bae, E., Geyer, P.K. Development (1995) [Pubmed]
  16. Genetic and molecular characterization of P element-induced mutations reveals that the Drosophila ovarian tumor gene has maternal activity and a variable null phenotype. Geyer, P.K., Patton, J.S., Rodesch, C., Nagoshi, R.N. Genetics (1993) [Pubmed]
WikiGenes - Universities