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Gene Review

Trf  -  TBP-related factor

Drosophila melanogaster

Synonyms: CG7562, Dmel\CG7562, TRF, TRF1, Trf1, ...
 
 
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High impact information on Trf

  • Transfection studies reveal that TRF can differentially mediate activation by some enhancer proteins but not others [1].
  • Reconstituted in vitro transcription reactions and deoxyribonuclease I footprinting assays confirmed the ability of TRF1 to bind preferentially and direct transcription of the tudor gene from an alternate promoter [2].
  • The TATA-binding protein (TBP)-related factor 1 (TRF1) is expressed in a tissue-restricted fashion during Drosophila embryogenesis and may serve as a promoter-specific recognition factor that can replace TBP in regulating transcription [2].
  • Our studies suggest that unlike most other eukaryotic organisms that rely on TBP for Pol III transcription, in Drosophila and possibly other insects the alternative TRF1/BRF complex appears responsible for the initiation of all known classes of Pol III transcription [3].
  • Here, we report the genome-wide identification of TRF1-binding sites using high-resolution genome tiling microarrays [3].
 

Biological context of Trf

  • Polytene chromosome staining, chromatin immunoprecipitation, direct messenger RNA analysis, and transient cotransfection assays identified the Drosophila gene tudor as containing a TRF1-responsive promoter [2].
  • The Drosophila TBP-related factor (TRF) is a neurogenesis-related transcription factor that binds to the TATA-box and activates transcription [4].
  • Furthermore, double-stranded RNA interference (dsRNAi) experiments were performed to ablate transcription factors such as TBP, TRF1, TRF2 and DREF in Schneider cells [5].
 

Other interactions of Trf

  • Novel TRF1/BRF target genes revealed by genome-wide analysis of Drosophila Pol III transcription [3].

References

  1. Transcription properties of a cell type-specific TATA-binding protein, TRF. Hansen, S.K., Takada, S., Jacobson, R.H., Lis, J.T., Tjian, R. Cell (1997) [Pubmed]
  2. Promoter-selective properties of the TBP-related factor TRF1. Holmes, M.C., Tjian, R. Science (2000) [Pubmed]
  3. Novel TRF1/BRF target genes revealed by genome-wide analysis of Drosophila Pol III transcription. Isogai, Y., Takada, S., Tjian, R., Kele??, S. EMBO J. (2007) [Pubmed]
  4. Identification of a mouse TBP-like protein (TLP) distantly related to the drosophila TBP-related factor. Ohbayashi, T., Makino, Y., Tamura, T.A. Nucleic Acids Res. (1999) [Pubmed]
  5. A DNA replication-related element downstream from the initiation site of Drosophila selenophosphate synthetase 2 gene is essential for its transcription. Jin, J.S., Baek, S., Lee, H., Oh, M.Y., Koo, Y.E., Shim, M.S., Kwon, S.Y., Jeon, I., Park, S.Y., Baek, K., Yoo, M.A., Hatfield, D.L., Lee, B.J. Nucleic Acids Res. (2004) [Pubmed]
 
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