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Gene Review:

coxII - Cytochrome c oxidase polypeptide II...

Rhodobacter sphaeroides 2.4.1

Cao, J. et al., Graichen, M.E. et al., Cao, J. et al.

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Disease relevance of coxII

The coxII/coxIII operon of Rhodobacter sphaeroides cytochrome c oxidase has been sequenced and characterized by insertional inactivation/complementation analysis [1].
A 2-kb fragment containing coxII DNA was subcloned into the phage M13mp18 and the sequence determined [2].
This study illustrates the potential utility of R. sphaeroides and the coxII promoter for heterologous expression of complex enzymes such as MADH which cannot be expressed in E. coli [3].

High impact information on coxII

Full recovery requires introduction into the bacterium of the complete operon containing coxII.orf1.orf3.coxIII; partial complementation yielding a spectrally altered enzyme is achieved with a plasmid containing coxII or coxII.orf1.orf3 [1].
The inactivation of coxII results in loss of the characteristic cytochrome oxidase spectrum from membranes of the mutant strain [1].
To accomplish the heterologous expression of correctly assembled MADH, eight genes from the methylamine utilization gene cluster of P. denitrificans, mauFBEDACJG, were placed under the regulatory control of the coxII promoter of Rhodobacter sphaeroides and introduced into R. sphaeroides by using a broad-host-range vector [3].

References

Cytochrome aa3 of Rhodobacter sphaeroides as a model for mitochondrial cytochrome c oxidase. The coxII/coxIII operon codes for structural and assembly proteins homologous to those in yeast. Cao, J., Hosler, J., Shapleigh, J., Revzin, A., Ferguson-Miller, S. J. Biol. Chem. (1992) [Pubmed]
The gene encoding cytochrome c oxidase subunit II from Rhodobacter sphaeroides; comparison of the deduced amino acid sequence with sequences of corresponding peptides from other species. Cao, J., Shapleigh, J., Gennis, R., Revzin, A., Ferguson-Miller, S. Gene (1991) [Pubmed]
Heterologous expression of correctly assembled methylamine dehydrogenase in Rhodobacter sphaeroides. Graichen, M.E., Jones, L.H., Sharma, B.V., van Spanning, R.J., Hosler, J.P., Davidson, V.L. J. Bacteriol. (1999) [Pubmed]

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