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Gene Review:

crtI - Phytoene dehydrogenase

Rhodobacter sphaeroides 2.4.1

Lang, H.P. et al., Garí, E. et al., Wang, C.W. et al.

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Disease relevance of crtI

Here we used directed evolution to change the product of Rhodobacter sphaeroides phytoene desaturase (crtI gene product), a neurosporene-producing enzyme, to lycopene [1].
The complete crtI gene has been overexpressed in Escherichia coli and R. sphaeroides and shown to catalyze three desaturations of phytoene to give neurosporene [2].

High impact information on crtI

Early steps in carotenoid biosynthesis: sequences and transcriptional analysis of the crtI and crtB genes of Rhodobacter sphaeroides and overexpression and reactivation of crtI in Escherichia coli and R. sphaeroides [2].

Biological context of crtI

In this report, we present the DNA and deduced amino acid sequences of the genes encoding these proteins, namely, crtB and crtI, from R. sphaeroides and present evidence for the existence of a crtIB operon [2].

Associations of crtI with chemical compounds

The N- and C-termini of these CrtD proteins present a high degree of similarity with the N- and C-termini of other carotenoid dehydrogenases including those encoded by crtI genes [3].

Other interactions of crtI

Although the crtI and crtB genes have been sequenced from a number of different organisms, the transcriptional organization and regulation of these genes have not been analyzed in detail [2].

References

Alteration of product specificity of Rhodobacter sphaeroides phytoene desaturase by directed evolution. Wang, C.W., Liao, J.C. J. Biol. Chem. (2001) [Pubmed]
Early steps in carotenoid biosynthesis: sequences and transcriptional analysis of the crtI and crtB genes of Rhodobacter sphaeroides and overexpression and reactivation of crtI in Escherichia coli and R. sphaeroides. Lang, H.P., Cogdell, R.J., Gardiner, A.T., Hunter, C.N. J. Bacteriol. (1994) [Pubmed]
Nucleotide sequence of the methoxyneurosporene dehydrogenase gene from Rhodobacter sphaeroides: comparison with other bacterial carotenoid dehydrogenases. Garí, E., Toledo, J.C., Gibert, I., Barbé, J. FEMS Microbiol. Lett. (1992) [Pubmed]

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