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Gene Review:

snRNA:U6:96Aa - small nuclear RNA U6 at 96Aa

Drosophila melanogaster

Synonyms: CR31379, DU6-1, Dmel\CR31379, K1, U6, ...

Das, G. et al., Takada, S. et al., Kiss, T. et al., Jensen, R.C. et al., Schimanski, B. et al., et al.

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Disease relevance of snRNA:U6:96Aa

The Drosophila U6 RNA genes were transcribed in vitro by Drosophila nuclear extracts but were not transcribed by Novikoff hepatoma or HeLa cell extracts [1].

High impact information on snRNA:U6:96Aa

A TATA box was found between nucleotides -23 and -31, and a stretch of 28 nucleotides from -43 to -71 was conserved in the 5'-flanking region of all three U6 RNA genes [1].
The Drosophila genome contains three U6 snRNA genes which are clustered in a 2-kilobase-pairs long DNA fragment [1].
The alteration of two or three base pairs near the 3'-end of the U1 and U6 PSEs was sufficient to switch the RNA polymerase specificity of Drosophila snRNA promoters in vitro [2].
One aspartic acid transfer RNA gene is present upstream of the U6 snRNA gene cluster in Drosophila melanogaster [3].
The timing of S.pombe U6 pre-RNA transport in the nucleus for splicing and methylation was also analyzed and is described [4].

Biological context of snRNA:U6:96Aa

A model is proposed for the base pairing interaction of broad bean U4 RNA with broad bean U6 RNA [5].

Analytical, diagnostic and therapeutic context of snRNA:U6:96Aa

Immunoprecipitation and in vitro transcription assays using immunodepleted extracts supplemented with recombinant proteins reveals that a TRF1:BRF complex is required to reconstitute transcription of tRNA, 5S and U6 RNA genes [6].
Recently, chromatin immunoprecipitation and a gel shift assay suggested that L. seymouri SNAP(c) also interacts with RNA pol III-transcribed U2 and U6 snRNA genes [7].

References

Structure, organization, and transcription of Drosophila U6 small nuclear RNA genes. Das, G., Henning, D., Reddy, R. J. Biol. Chem. (1987) [Pubmed]
The proximal sequence element (PSE) plays a major role in establishing the RNA polymerase specificity of Drosophila U-snRNA genes. Jensen, R.C., Wang, Y., Hardin, S.B., Stumph, W.E. Nucleic Acids Res. (1998) [Pubmed]
One aspartic acid transfer RNA gene is present upstream of the U6 snRNA gene cluster in Drosophila melanogaster. Das, G., Henning, D., Reddy, R. Nucleic Acids Res. (1986) [Pubmed]
The Schizosaccharomyces pombe mgU6-47 gene is required for 2'-O-methylation of U6 snRNA at A41. Zhou, H., Chen, Y.Q., Du, Y.P., Qu, L.H. Nucleic Acids Res. (2002) [Pubmed]
Plant small nuclear RNAs. V. U4 RNA is present in broad bean plants in the form of sequence variants and is base-paired with U6 RNA. Kiss, T., Jakab, G., Antal, M., Pálfi, Z., Hegyi, H., Kis, M., Solymosy, F. Nucleic Acids Res. (1988) [Pubmed]
A TRF1:BRF complex directs Drosophila RNA polymerase III transcription. Takada, S., Lis, J.T., Zhou, S., Tjian, R. Cell (2000) [Pubmed]
Failure to detect binding of Trypanosoma brucei SNAPc to U2 and U6 snRNA gene sequences by in vitro transcription competition and pull-down assays. Schimanski, B., Ma, L., Günzl, A. Mol. Biochem. Parasitol. (2004) [Pubmed]

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