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Gene Review:

ebpS - cell surface elastin binding protein

Staphylococcus aureus RF122

Smeltzer, M.S. et al., Park, P.W. et al., Downer, R. et al., Vancraeynest, D. et al.

Vancraeynest, Hermans, Haesebrouck, Smeltzer, Gillaspy, Pratt, Thames, Iandolo,

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Disease relevance of ebpS

Molecular cloning and expression of the gene for elastin-binding protein (ebpS) in Staphylococcus aureus [1].

High impact information on ebpS

DNA sequence data indicate that the ebpS open reading frame consists of 606 base pairs and encodes a novel polypeptide with a predicted molecular mass of 23,345 daltons and pI of 4 [1].
Using Southern blot analysis of SmaI-digested genomic DNA resolved by pulsed-field gel electrophoresis, the adhesion genes were mapped to SmaI fragments A (ebpS), B (fib and clfA), C (fnbA/fnbB), E (fbpA), F (map) and G (cna) [2].
All 25 strains encoded fib, clfA, ebpS, map and at least one of the fnb genes. fbpA and coa appeared to be allelic variants of the same gene with the fbpA variant being present in only four of 25 isolates. cna was present in 10 of 25 strains [2].

Biological context of ebpS

The prevalences of ebpS, eno, fnbA and fib did not reveal striking differences between high and low virulence strains [3].

Analytical, diagnostic and therapeutic context of ebpS

Western blotting of lysates of wild-type 8325-4 and Newman and the corresponding ebpS mutants showed that EbpS migrated with an apparent molecular mass of 83 kDa [4].

References

Molecular cloning and expression of the gene for elastin-binding protein (ebpS) in Staphylococcus aureus. Park, P.W., Rosenbloom, J., Abrams, W.R., Rosenbloom, J., Mecham, R.P. J. Biol. Chem. (1996) [Pubmed]
Prevalence and chromosomal map location of Staphylococcus aureus adhesin genes. Smeltzer, M.S., Gillaspy, A.F., Pratt, F.L., Thames, M.D., Iandolo, J.J. Gene (1997) [Pubmed]
Genotypic and phenotypic screening of high and low virulence Staphylococcus aureus isolates from rabbits for biofilm formation and MSCRAMMs. Vancraeynest, D., Hermans, K., Haesebrouck, F. Vet. Microbiol. (2004) [Pubmed]
The elastin-binding protein of Staphylococcus aureus (EbpS) is expressed at the cell surface as an integral membrane protein and not as a cell wall-associated protein. Downer, R., Roche, F., Park, P.W., Mecham, R.P., Foster, T.J. J. Biol. Chem. (2002) [Pubmed]

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