Disease relevance of polC

• Development of a universal probe for electronic microarray and its application in characterization of the Staphylococcus aureus polC gene [1].
• The polC gene encodes PolC, another catalytic subunit of DNA polymerase, which is specifically found in gram-positive bacteria [2].
• Monoclonal antibodies specific for the alpha subunit of the Escherichia coli DNA polymerase III holoenzyme [3].

High impact information on polC

• We have established three murine hybridoma cell lines that secrete monoclonal antibodies directed specifically against the alpha subunit of the DNA polymerase III holoenzyme of Escherichia coli [3].
• DNA sequence analysis of the entire polC genes from both wild-type and resistant organisms revealed that the resistant organisms had a sequence change that mapped to a single amino acid codon in all strains examined [4].
• A new class of 6-anilinouracils based on N-3 alkyl substitution of the uracil ring was synthesized and analyzed for activity as inhibitors of the gram-positive bacterial DNA polymerase III and the growth of gram-positive bacterial pathogens [5].
• The re-PolIII was purified to > 98% homogeneity and was shown by N-terminal amino acid sequence analysis to be the bona fide product of the Sa polC ORF [6].
• The polC gene mapped between omega 1074 [Tn551] and recA/ngr on the Sa NCTC 8325 genome [6].

Biological context of polC

• The feasibility of this approach was demonstrated by successfully analyzing eight SNPs distributed within a highly variable 1-kb region of the polC gene from six isolates of Staphylococcus aureus [1].
• The polC gene specifying DNA polymerase III (PolIII) of Staphylococcus aureus (Sa), was cloned with a novel strategy and found to contain a 4305-bp open reading frame (ORF) encoding a polypeptide of approx. 162 kDa [6].

References