Disease relevance of CG14906

• Here we show that, in contrast to 17.6 ORF2, ORFs of copia have no extensive amino-acid sequence homology to the RT region of the reverse transcriptase of retrovirus in vertebrates [1].
• Direct N terminus sequence analysis of two capsid proteins showed that ORF2 (nt 6109-8715) encodes the structural proteins of TrV [2].

High impact information on CG14906

• The complete I factor sequence contains two long open reading frames, ORF1 and ORF2, of 1278 and 3258 bp [3].
• ORF1 encodes a possible nucleic acid-binding protein, and part of the amino acid sequence of ORF2 is similar to that of viral reverse transcriptases and polypeptides encoded by L1 elements [3].
• ORF2 showed more substantial homology to retroviral pol genes and particularly to the analogs of pol found in a subclass of mobile DNAs that are supposed retrotransposons, such as mammalian long interspersed repetitive sequences, Drosophila I factors, silkworm R1 elements, and trypanosome Ingi elements [4].
• The intergenic UTR is known to contain an internal ribosome entry site (IRES) which directs non-AUG-initiated translation of ORF2 [5].
• The Cili-1 sequences show matches to regions of the ORF2 product of mammalian L1 elements [6].

Biological context of CG14906

• A complete gypsyDS sequence was isolated from a D. subobscura genomic library, and a 1.3-kb fragment which aligns with the ORF2 of the D. melanogaster gypsy element was sequenced [7].
• The site of initiation of protein synthesis for ORF2 could not be determined from the amino acid and nucleotide sequences [8].
• The predicted amino acid sequence of ORF2 is very similar to the corresponding regions of Drosophila C virus, Plautia stali intestine virus, and to a partial sequence from the 3' end of the cricket paralysis virus genome [8].
• Judging by the sequence homologies, ORF1 codes for a nucleic-acid-binding protein, and ORF2 for a reverse transcriptase which is most similar in its sequence to putative reverse transcriptase of other LINEs [9].
• It was shown that the previously demonstrated variations in retrotranspositional activity are associated with either one or both of two amino acid substitutions at the beginning of ORF2 [10].

Anatomical context of CG14906

• This paper shows that IviP2 can be mobilized more efficiently in the germ line of strongly reactive females in the absence of active I factors, when it is trans-complemented by the product of ORF2 synthesized from the hsp70 heat-shock promoter [11].

References