Disease relevance of MECR

• Comparison of the predicted peptide sequence of pSR132 with other proteins compiled in the NBRF data base revealed significant homology with carboxyphosphonoenolpyruvate mutase and phosphoenolpyruvate mutase from Streptomyces hygroscopicus and Tetrahymena pyriformis, respectively [1].

High impact information on MECR

• Neither the DNA sequence determined from a full-length cDNA clone overlapping with clone AH2-519 nor the amino acid sequence of its predicted protein product has significant homology to published sequences in the GenBank or NBRF databases [2].
• Expression of HsNrbf-1, tagged C-terminally with green fluorescent protein, in HeLa cells, resulted in a punctated fluorescence signal, superimposable with the MitoTracker Red dye [3].
• Here, some structural aspects of proline kinks in transmembrane helices are discussed on the basis of an analysis of Pro-kinked helices in the photosynthetic reaction center and bacteriorhodopsin, as well as results from an analysis of Pro-containing transmembrane segments identified in the NBRF Protein Sequence Databank [4].
• The open reading frame present in GOS5 codes for a protein (15 kDa) that contains a putative chloroplast transit peptide and does not show any significant homology to protein sequences in the NBRF protein database [5].
• This server allows to query nucleotide sequence banks in the EMBL/GenBank/DDBJ formats and protein sequence banks in the NBRF/PIR format [6].

Biological context of MECR

• A search for homologies in a protein database (NBRF) revealed that the predicted protein product has a functional peptide domain that resembles that of 2-oxoglutarate-dependent dioxygenases [7].

Anatomical context of MECR

• This gene is expressed predominantly in thymocytes and encodes a newly identified intracytoplasmic protein specific for T cells, since neither the nucleotide sequence of the cDNA clone nor the deduced amino acid sequence has significant homology to sequences in the GenBank or NBRF databases [8].

References