High impact information on RAB23

• Rab23, a negative regulator of hedgehog signaling, localizes to the plasma membrane and the endocytic pathway [1].
• HCC cell lines expressed Rab23 [2].
• Knocking down rab23 suppressed Hep3B cell growth, suggesting that rab23 could play an important role in Hep3B cell growth [2].
• The biological significance of Rab23 in Hep-3B cells was examined by knocking down Rab23 gene expression [2].
• However the absence of pathogenic variations after molecular analysis of the coding sequence in the index patients of RP25 linked families would be consistent with the exclusion of RAB23 as responsible for RP25 phenotype [3].

Analytical, diagnostic and therapeutic context of RAB23

• Molecular cloning and characterization of human RAB23, a member of the group of Rab GTPases [3].
• In this study, the localization of Rab23 was analyzed by light and immunoelectron microscopy after expression of wild-type (Rab23-GFP), constitutively active Rab23 (Rab23Q68L-GFP), and inactive Rab23 (Rab23S23N-GFP) in a range of mammalian cell types [1].
• METHODS: Primary tumors (n = 100) were stained with Rab23 antibodies using immunohistochemistry and in situ hybridization in tissue microarrays [2].
• Rab23 expression in these cells was examined using RT-PCR and Western blots [2].

References