High impact information on ROPN1

• These results suggest that rhophilin and ropporin may form a complex in sperm flagella [1].
• The amino-terminal 40 amino acid sequence of ropporin showed high homology to that of the regulatory subunit of type II cAMP-dependent protein kinase, which is involved in dimerization and binding to A-kinase anchoring proteins [1].
• Northern blot analysis showed that ropporin is exclusively expressed in testis, and induced at the late stage of spermatogenesis [1].
• The open reading frame of the cDNA encoded a novel protein of 212 amino acids designated as ropporin from a Japanese word 'oppo' (the tail) [1].

Analytical, diagnostic and therapeutic context of ROPN1

• Consistently, a yeast two hybrid assay and gel filtration of recombinant ropporin indicated that ropporin dimerizes through this domain [1].
• Immunocytochemistry using anti-ropporin antibody showed that ropporin is localized in the principal piece and the end piece of sperm flagella [1].

References