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Gene Review

rps12  -  ribosomal protein S12

Nicotiana tabacum

 
 
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Disease relevance of rps12

 

High impact information on rps12

  • RNA gel blot hybridization analysis and primer extension sequencing of cDNA to rps12 encoding RNAs indicate that exon 1 and exons 2 and 3 are encoded on separate transcripts [1].
  • These results indicate that a bimolecular (trans-) splicing event occurs during the formation of mature rps12 mRNA [1].
  • C-terminal exons 2 and 3 of rps12 are located in the inverted repeat regions of the tobacco chloroplast genome [1].
  • Exon 1 and exons 2 and 3 are covalently ligated in the correct reading frame in rps12 mRNA [1].
  • In E. coli, alteration in rps12 codons 42 or 87 causes streptomycin resistance [2].
 

Biological context of rps12

 

Anatomical context of rps12

  • We report that a streptomycin-resistant mutant isolated from protoplast cultures of N. plumbaginifolia contains an A-to-G transition at nucleotide position 149 in exon 2 of the chloroplast rps12 gene [3].
  • Based on data showing a common sequence in mitochondria and chloroplast DNA of maize (Stern and Lonsdale 1982) and that the rps12 gene of tobacco chloroplast DNA is a divided gene (Torazawa et al. 1986), it is suggested that the sequence on the inverted repeat structure of chloroplast DNA may have the character of a movable genetic element [4].
 

Analytical, diagnostic and therapeutic context of rps12

  • However, the 5' 37 codon of Nicotiana chloroplast rps12 could not be identified by electron microscopy of RNA-DNA hybrids within a DNA region extending 4000 bp upstream of codon 38, nor by computer search of a completely sequenced region extending for more than 9000 bp upstream of this codon [2].

References

  1. Trans-splicing in chloroplasts: the rps 12 loci of Nicotiana tabacum. Hildebrand, M., Hallick, R.B., Passavant, C.W., Bourque, D.P. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  2. The enigma of the gene coding for ribosomal protein S12 in the chloroplasts of Nicotiana. Fromm, H., Edelman, M., Koller, B., Goloubinoff, P., Galun, E. Nucleic Acids Res. (1986) [Pubmed]
  3. A point mutation in the chloroplast rps12 gene from Nicotiana plumbaginifolia confers streptomycin resistance. Hsu, C.M., Yang, W.P., Chen, C.C., Lai, Y.K., Lin, T.Y. Plant Mol. Biol. (1993) [Pubmed]
  4. Variable copy number DNA sequences in rice. Kikuchi, S., Takaiwa, F., Oono, K. Mol. Gen. Genet. (1987) [Pubmed]
 
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