High impact information on HIST1H4F

• Similar results were found in HeLa S3 and HL60 cells for the cell cycle-dependent human H4 histone gene FO108 [1].
• The proliferation-specific transcription factor complex HiNF-D interacts with sequence specificity in a proximal promoter element of the human H4 histone gene FO108, designated Site II [2].
• Downregulation of transcription from the H4 gene occurs concomitant with the loss of the HiNF-D/Site II interaction, further suggesting a functional relationship between Site II occupancy and the capacity for transcription [2].
• To test for regulatory elements within the coding region, we performed transient transfection experiments in HeLa cells using constructs with histone H4 sequences fused upstream of a heterologous thymidine kinase promoter and CAT reporter gene [3].
• Our results indicate that the proximal 200 nucleotides of the histone H4-coding region contain transcriptional regulatory elements that may contribute to cell-cycle control of histone gene expression by interacting with repressor complexes containing CDP/cut homeodomain transcription factors [3].

Biological context of HIST1H4F

• The coding region of the human histone H4 gene FO108 undergoes dynamic changes in chromatin structure that correlate with modifications in gene expression [3].
• Upon analysis of the regulatory elements of the FO108 histone H4 gene, we identified several potential YY1 binding sites [4].

References