Disease relevance of Sytl2

• Slp2 and Rab27A were distributed to the periphery of the cells (especially at the dendritic tips) in the wild-type melanoma cells, whereas they accumulated in the perinuclear region in the melanosome transport-defective cells (S91/Cloudman) [1].

High impact information on Sytl2

• Rab27A-binding protein Slp2-a is required for peripheral melanosome distribution and elongated cell shape in melanocytes [2].
• Loss of Slp2-a protein also induced a change in melanocyte morphology, from their normal elongated shape to a more rounded shape, which depended on the phospholipid-binding activity of Slp2-a, but not on its Rab27A-binding activity [2].
• Immunocytochemical analysis revealed that Slp2 (or Slp1) colocalized with Rab27A in the melanosomes of melanoma cells [1].
• However, the precise tissue distribution of Slp2-a protein and its functions remain largely unknown [3].
• Analysis of Slp2-a mutant mice generated by homologous recombination showed a reduced number of mucus granules, a deficiency of granule docking with the apical plasma membrane in the gastric-surface mucous cells and reduction of mucus secretion by Slp2-a-deficient gastric primary cells [3].

Biological context of Sytl2

• We presently show that activation of murine T-cells in presence of these immunosuppressors results in the up-regulation of the synaptotagmin-like 2 gene [4].
• Slp2-f has an open reading frame coding for a putative protein of 1229 amino acids sharing 47% identities with the human breast-associated antigen, SGA-72 M. In addition to the well-documented modulation of gene transcription, the two immunosuppressors also play a role in the choice of alternative splice sites on murine Slp2 pre-mRNA [4].

Anatomical context of Sytl2

• Knockdown of endogenous Slp2-a protein by small-interfering RNAs (siRNAs) markedly reduced the number of melanosomes in the cell periphery of mouse melanocytes ('peripheral dilution') [2].

Associations of Sytl2 with chemical compounds

• The usage of alternative splice sites in mus musculus synaptotagmin-like 2 gene is modulated by cyclosporin A and FK506 in T-lymphocytes [4].

Analytical, diagnostic and therapeutic context of Sytl2

• Co-immunoprecipitation experiments indicated that Slp2-a interacts with Rab27A/B in vivo [3].

References