Disease relevance of MCM8

• In a case of choriocarcinoma MCM8 mRNA is aberrant, leading to expression of a protein lacking 16 amino acids [1].
• In several cases of colon adenocarcinoma MCM8 expression is greatly reduced relative to matched non-cancerous tissue [1].

High impact information on MCM8

• Furthermore, down-regulation of hMCM8 also leads to a reduced loading of hcdc6 and the hMCM2-hMCM7 complex on chromatin [2].
• Depletion of endogenous hMCM8 protein by RNA interference leads to a delay of entry into S phase, suggesting a role for hMCM8 in G(1) progression [2].
• Chromatin immunoprecipitation (ChIP) demonstrated the direct association of E2F1 and NF-Y with human MCM8 promoter [3].
• Here we characterized MCM8 orthologous genes by using bioinformatics [3].
• Analysis of human MCM8 promoter constructs showed that an E2F-binding motif in the vicinity of the transcription initiation site is necessary for the transcriptional activation [3].

Biological context of MCM8

• The MCM8 gene, consisting of 19 exons, is located contrapodal to a gene, consisting of 11 exons, encoding a homolog of the yeast GCD10 gene product [1].
• A new member of the MCM protein family encoded by the human MCM8 gene, located contrapodal to GCD10 at chromosome band 20p12.3-13 [1].
• Like MCM8, MCM9 is only present in the genome of higher eukaryotes [4].

References