High impact information on NRG2

• Deletion of NRG1 (but not of NRG2) resulted in increased expression [1].
• We identified Nrg2 as a protein that interacts with Snf1 in the two-hybrid system [2].
• A LexA fusion to Nrg2 represses transcription from a promoter containing LexA binding sites, indicating that Nrg2 also functions as a repressor [2].
• In addition, three intronic single nucleotide polymorphisms were identified in the NRG2 gene [3].
• The NRG2 gene was found to be on two yeast artificial chromosomes overlapping the candidate interval and was, thus, considered a good positional candidate for this form of CMT [3].

Biological context of NRG2

• In accord with these findings, mutation of NRG1 and NRG2 enhanced the resistance of cells to salt and oxidative stress and decreased tolerance to freezing [4].
• The Nrg1 and Nrg2 repressors of Saccharomyces cerevisiae have highly similar zinc fingers and closely related functions in the regulation of glucose-repressed genes [5].

Associations of NRG2 with chemical compounds

• Expression of NRG1 RNA is glucose repressed, whereas NRG2 RNA levels are nearly constant [5].

Analytical, diagnostic and therapeutic context of NRG2

• Chromatin immunoprecipitation assays showed that Nrg1 and Nrg2 bind DNA both in the presence and absence of glucose [5].
• By using radiation hybrid mapping panels, the human NRG2 gene was mapped to the D5S658-D5S402 region within 5q23-q33, close to an autosomal recessive form of demyelinating Charcot-Marie-Tooth (CMT) disease [3].

References