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Gene Review

NRG1  -  Nrg1p

Saccharomyces cerevisiae S288c

Synonyms: MSS1, Transcriptional regulator NRG1, YD5112.01C, YDR043C, Zinc finger protein MSS1
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High impact information on NRG1

  • We show that growth in serum at 37 degrees C, a potent inducer of filamentous growth, causes a reduction of NRG1 mRNA, suggesting that filamentous growth is induced by the down-regulation of NRG1 [1].
  • These genes constitute a subset of those under Tup1 control, providing further evidence that Nrg1 acts by recruiting Tup1 to target genes [1].
  • We show that Nrg1 and Rim101 bind simultaneously to adjacent target sites within the NRE in vitro and act as corepressors in vivo [2].
  • NRG1 and SFL1 expression requires the Srb8-11 complex, and correspondingly, the Srb8-11 complex is also necessary for STA1 repression [3].
  • Glucose repression of STA1 expression is mediated by the Nrg1 and Sfl1 repressors and the Srb8-11 complex [3].

Biological context of NRG1

  • The NRG1 gene encodes a 25-kDa C2H2 zinc finger protein which specifically binds to two regions in the upstream activation sequence of the STA1 gene, as judged by gel retardation and DNase I footprinting analyses [4].
  • In accord with these findings, mutation of NRG1 and NRG2 enhanced the resistance of cells to salt and oxidative stress and decreased tolerance to freezing [5].
  • Deletion mutations of the Rim101p repression targets NRG1 and SMP1 suppress rim101Delta mutant defects in ion tolerance, haploid invasive growth, and sporulation [6].
  • The transcription factor Rim101p governs ion tolerance and cell differentiation by direct repression of the regulatory genes NRG1 and SMP1 in Saccharomyces cerevisiae [6].
  • Previously, we found that expression of the C-terminally truncated form of NRG1 (NRG1(1-470)) on a multicopy plasmid increases the hydrophobicity of the cell surface, conferring flor formation on the non-flor laboratory strain [7].

Associations of NRG1 with chemical compounds

  • The expression of NRG1 itself is inhibited in the absence of glucose [4].
  • Two-hybrid and glutathione S-transferase pull-down experiments show an interaction of Nrg1 with Ssn6 both in vivo and in vitro [4].
  • Disruption of the Nrg1p-repressed gene FLO11, which encodes a cell surface glycoprotein that functions as a flocculin or an adhesin, abolished flor formation [8].

Other interactions of NRG1

  • The Mig1-independent role depends on Nrg1p [9].
  • Expression of NRG1 RNA is glucose repressed, whereas NRG2 RNA levels are nearly constant [10].
  • Deletion of the NRG1 and NRG2 genes suppressed the defects of a snf1 mutant in all of these processes [11].
  • A key target is the gene ENA1, encoding a Na(+)-ATPase, whose induction by alkaline pH has been shown to involve calcineurin and the Rim101/Nrg1 pathway [12].
  • Disruption of the NRG1 gene causes a fivefold increase in the level of the STA1 transcript in the presence of glucose [4].

Analytical, diagnostic and therapeutic context of NRG1


  1. NRG1, a repressor of filamentous growth in C.albicans, is down-regulated during filament induction. Braun, B.R., Kadosh, D., Johnson, A.D. EMBO J. (2001) [Pubmed]
  2. Components of the ESCRT pathway, DFG16, and YGR122w are required for Rim101 to act as a corepressor with Nrg1 at the negative regulatory element of the DIT1 gene of Saccharomyces cerevisiae. Rothfels, K., Tanny, J.C., Molnar, E., Friesen, H., Commisso, C., Segall, J. Mol. Cell. Biol. (2005) [Pubmed]
  3. Glucose repression of STA1 expression is mediated by the Nrg1 and Sfl1 repressors and the Srb8-11 complex. Kim, T.S., Lee, S.B., Kang, H.S. Mol. Cell. Biol. (2004) [Pubmed]
  4. Nrg1 is a transcriptional repressor for glucose repression of STA1 gene expression in Saccharomyces cerevisiae. Park, S.H., Koh, S.S., Chun, J.H., Hwang, H.J., Kang, H.S. Mol. Cell. Biol. (1999) [Pubmed]
  5. Repressors Nrg1 and Nrg2 regulate a set of stress-responsive genes in Saccharomyces cerevisiae. Vyas, V.K., Berkey, C.D., Miyao, T., Carlson, M. Eukaryotic Cell (2005) [Pubmed]
  6. The transcription factor Rim101p governs ion tolerance and cell differentiation by direct repression of the regulatory genes NRG1 and SMP1 in Saccharomyces cerevisiae. Lamb, T.M., Mitchell, A.P. Mol. Cell. Biol. (2003) [Pubmed]
  7. FLO11 is the primary factor in flor formation caused by cell surface hydrophobicity in wild-type flor yeast. Ishigami, M., Nakagawa, Y., Hayakawa, M., Iimura, Y. Biosci. Biotechnol. Biochem. (2006) [Pubmed]
  8. FLO11 is essential for flor formation caused by the C-terminal deletion of NRG1 in Saccharomyces cerevisiae. Ishigami, M., Nakagawa, Y., Hayakawa, M., Iimura, Y. FEMS Microbiol. Lett. (2004) [Pubmed]
  9. MIG1-dependent and MIG1-independent regulation of GAL gene expression in Saccharomyces cerevisiae: role of Imp2p. Alberti, A., Lodi, T., Ferrero, I., Donnini, C. Yeast (2003) [Pubmed]
  10. Nrg1 and nrg2 transcriptional repressors are differently regulated in response to carbon source. Berkey, C.D., Vyas, V.K., Carlson, M. Eukaryotic Cell (2004) [Pubmed]
  11. Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Kuchin, S., Vyas, V.K., Carlson, M. Mol. Cell. Biol. (2002) [Pubmed]
  12. The Transcriptional Response of the Yeast Na+-ATPase ENA1 Gene to Alkaline Stress Involves Three Main Signaling Pathways. Platara, M., Ruiz, A., Serrano, R., Palomino, A., Moreno, F., Ari??o, J. J. Biol. Chem. (2006) [Pubmed]
  13. Cloning and characterisation of a glucoamylase gene (GlaM) from the dimorphic zygomycete Mucor circinelloides. Houghton-Larsen, J., Pedersen, P.A. Appl. Microbiol. Biotechnol. (2003) [Pubmed]
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