Disease relevance of CCH1

• Here, we report the successful cloning of CCH1 in yeast by in vivo homologous recombination without using E. coli [1].

High impact information on CCH1

• To counteract the cation chelating activity of citric acid, the plasma membrane Ca(2+) channel, CCH1, and a functional vacuolar membrane H(+)-ATPase were found to be essential for optimal adaptation [2].
• A screen for yeast mutants specifically defective in the high-affinity Ca(2+) uptake system revealed two genes, CCH1 and MID1, previously implicated in Ca(2+) influx in response to mating pheromones [3].
• Expression of TaTPC1 in the yeast mutant lacking CCH1 can recover its growth under lithium stress through functional complementation [4].
• The K(m) value for Ca(2+) of OsTPC1, 47.5 micro M, was comparable to that of intrinsic CCH1, a candidate VDCC in yeast [5].

Anatomical context of CCH1

• The gene (CCH1) corresponding to the second suppressor encodes a protein that bears significant sequence similarity to the pore-forming subunit (alpha1) of plasma membrane, voltage-gated Ca2+ channels from higher eukaryotes [6].

Analytical, diagnostic and therapeutic context of CCH1

• Saccharomyces cerevisiae has only one gene encoding a putative voltage-gated Ca2+ channel pore-forming subunit, CCH1, which is not possible to be cloned by conventional molecular cloning techniques using Escherichia coli [1].

References