Disease relevance of BIO2

• Analysis of the yeast protein encoded by the BIO2 gene reveals that it shares extensive homology with biotin synthases of E. coli and Bacillus sphaericus [1].

High impact information on BIO2

• Expression of BIO2 was higher in the light relative to dark and was induced 5-fold during biotin-limited conditions [2].
• Comparisons of the deduced amino acid sequence from BIO2 with bacterial and yeast biotin synthase homologs revealed a high degree of sequence similarity [2].
• Although BIO2 is physically linked to ZUO1, which encodes the putative left-handed Z-DNA binding protein zuotin, it appears to be regulated independently from it [1].
• The plasmid pUCBIO2 containing the yeast BIO2 gene completely complements E. coli bioB- and delta bio mutants and enables these mutants to grow on dethiobiotin [1].
• A series of plasmids were constructed to direct the integration of the BIO2 gene, either high-copy integration with 18S rDNA fragment or low-copy integration with URA3 or HIS3 fragment [3].

Biological context of BIO2

• The BIO2 gene can be successfully integrated into the C. utilis chromosome and can drive biotin production using these plasmids [3].

Associations of BIO2 with chemical compounds

• BIO2 is the first eukaryotic gene reported from the biotin biosynthetic pathway [1].

References