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Gene Review

bioH  -  pimeloyl-ACP methyl ester carboxylesterase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3399, JW3375, bioB
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Disease relevance of bioH

  • The bioI gene encodes a protein with similarity to cytochrome P-450s and was able to complement mutations in either bioC or bioH of E. coli [1].
  • The Serratia marcescens bioH gene encodes an esterase [2].
  • To investigate this further, we have overexpressed and purified the bioH gene products from both E. coli (BIOH EC) and Neisseria meningitis (BIOH NM) in E. coli [3].

High impact information on bioH

  • Various nonorthologous substitutes of the bioC-coupled gene bioH from E. coli, observed in several genomes, possibly represent the existence of different pathways for pimeloyl-CoA biosynthesis [4].
  • Nucleotide sequence of the bioH gene of Escherichia coli [5].
  • The 104-kDa protein can be converted to the 82-kDa protein upon incubation with Fe3+ and S2-. The bioB gene product we have isolated is active in the conversion of dethiobiotin to biotin in vitro in the presence of NADPH, AdoMet, Fe3+ or Fe2+, and additional unidentified factors from the crude extracts of E. coli [6].
  • Interestingly, the ORF was 70% identical to a product of the E. coli bioH gene, which lies at a locus separated from the bioABFCD operon and acts in the early steps of the biotin synthetic pathway before pimeloyl-CoA synthesis [2].
  • Although the enzymatic function of BioH is not yet elucidated, the bioH gene products from S. marcescens and E. coli show esterase activity, which may imply the hydrolysis of a precursor leading to pimeloyl-CoA ester [2].

Chemical compound and disease context of bioH


Biological context of bioH


Associations of bioH with chemical compounds

  • Eleven biotin biosynthetic genes have been identified in Kurthia sp. Kurthia sp. has two genes coding for KAPA synthase, bioF and bioFII, and also has two genes coding for BioH protein, bioH and bioHII [11].
  • Our results suggest that an unidentified enzyme(s) besides the bioB gene product is obligatory for the conversion of dethiobiotin to biotin [10].

Enzymatic interactions of bioH


Other interactions of bioH

  • The B. sphaericus bioD and bioA genes were unambiguously identified within the 4.3-kb insert and shown to be closely linked to bioY (coding for a protein with a presently unknown function) and to bioB [Ohsawa et al., Gene 80 (1989) 39-48] [7].
  • In Escherichia coli, biotin synthase is coded for by bioB gene [12].


  1. Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon. Bower, S., Perkins, J.B., Yocum, R.R., Howitt, C.L., Rahaim, P., Pero, J. J. Bacteriol. (1996) [Pubmed]
  2. The Serratia marcescens bioH gene encodes an esterase. Akatsuka, H., Kawai, E., Sakurai, N., Omori, K. Gene (2003) [Pubmed]
  3. Purification and characterisation of the BIOH protein from the biotin biosynthetic pathway. Tomczyk, N.H., Nettleship, J.E., Baxter, R.L., Crichton, H.J., Webster, S.P., Campopiano, D.J. FEBS Lett. (2002) [Pubmed]
  4. Conservation of the biotin regulon and the BirA regulatory signal in Eubacteria and Archaea. Rodionov, D.A., Mironov, A.A., Gelfand, M.S. Genome Res. (2002) [Pubmed]
  5. Nucleotide sequence of the bioH gene of Escherichia coli. O'Regan, M., Gloeckler, R., Bernard, S., Ledoux, C., Ohsawa, I., Lemoine, Y. Nucleic Acids Res. (1989) [Pubmed]
  6. Biotin synthase: purification, characterization as a [2Fe-2S]cluster protein, and in vitro activity of the Escherichia coli bioB gene product. Sanyal, I., Cohen, G., Flint, D.H. Biochemistry (1994) [Pubmed]
  7. Cloning and characterization of the Bacillus sphaericus genes controlling the bioconversion of pimelate into dethiobiotin. Gloeckler, R., Ohsawa, I., Speck, D., Ledoux, C., Bernard, S., Zinsius, M., Villeval, D., Kisou, T., Kamogawa, K., Lemoine, Y. Gene (1990) [Pubmed]
  8. Biotin synthesis in higher plants: purification and characterization of bioB gene product equivalent from Arabidopsis thaliana overexpressed in Escherichia coli and its subcellular localization in pea leaf cells. Baldet, P., Alban, C., Douce, R. FEBS Lett. (1997) [Pubmed]
  9. Functional analysis of Sinorhizobium meliloti genes involved in biotin synthesis and transport. Entcheva, P., Phillips, D.A., Streit, W.R. Appl. Environ. Microbiol. (2002) [Pubmed]
  10. Conversion of dethiobiotin to biotin in cell-free extracts of Escherichia coli. Ifuku, O., Kishimoto, J., Haze, S., Yanagi, M., Fukushima, S. Biosci. Biotechnol. Biochem. (1992) [Pubmed]
  11. Cloning and characterization of biotin biosynthetic genes of Kurthia sp. Kiyasu, T., Nagahashi, Y., Hoshino, T. Gene (2001) [Pubmed]
  12. The gene for biotin synthase from Saccharomyces cerevisiae: cloning, sequencing, and complementation of Escherichia coli strains lacking biotin synthase. Zhang, S., Sanyal, I., Bulboaca, G.H., Rich, A., Flint, D.H. Arch. Biochem. Biophys. (1994) [Pubmed]
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