Disease relevance of ZUO1

• The hexapeptide motif KYHPDK was found in zuotin as well as in several yeast proteins, DnaJ of E.coli, csp29 and csp32 proteins of Drosophila and the small t and large T antigens of the polyoma virus [1].

Psychiatry related information on ZUO1

• MIDA1 associates with the HLH region of Id with the conserved region adjacent to eukaryotic DnaJ conserved motif within the Zuotin homology region, although it does not have any canonical HLH motif [2].

High impact information on ZUO1

• Analysis of zuo1 deletion and truncation mutants revealed a positive correlation between the ribosome association of Zuo1 and its ability to bind RNA [3].
• Here we report that the phenotypes of an S.cerevisiae strain lacking the DnaJ-related protein Zuotin (Zuo1) are very similar to those of a strain lacking Ssb, including sensitivities to low temperatures, certain protein synthesis inhibitors and high osmolarity [3].
• Zuo1, which has been shown previously to be a nucleic acid-binding protein, is also a ribosome-associated protein localized predominantly in the cytosol [3].
• Poly(dG-Br5dC) in the Z-form competed well for the binding of a zuotin containing fraction, but salmon sperm DNA, poly(dG-dC) and poly(dA-dT) were not effective [1].
• However, a 50- to 100-fold reduction of Ssz1 and Zuo1 levels does not have a substantial effect on cell growth [4].

Chemical compound and disease context of ZUO1

• This protein is named MIDA1 (Mouse Id Associate 1), and its predicted amino acid sequence consists of Zuotin (a putative Z-DNA binding protein in yeast) homology region and tryptophan-mediated repeats similar to c-Myb oncoprotein [2].

Biological context of ZUO1

• Disruption of ZUO1 in yeast resulted in a slow growth phenotype [1].
• It consists of a 355-codon open reading frame near the ZUO1 gene [5].
• The yeast BIO2 and ZUO1 genes reside near ADE3 gene on chromosome VII [5].
• The DnaJ domain and candidate phosphorylation sites of zuotin and ZRF1 are highly conserved [6].
• Although BIO2 is physically linked to ZUO1, which encodes the putative left-handed Z-DNA binding protein zuotin, it appears to be regulated independently from it [5].

Anatomical context of ZUO1

• In addition, Pdr13p co-sedimented with translating ribosomes and this association was independent of the presence of Zuo1p [7].

Other interactions of ZUO1

• ZUO1 encodes a DnaJ-related Hsp40 [7].
• Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene [8].
• We previously identified a zuo1 mutation as an extragenic suppressor of the tom1 mutant [7].
• Activation of pleiotropic drug resistance by the J-protein and Hsp70-related proteins, Zuo1 and Ssz1 [9].

Analytical, diagnostic and therapeutic context of ZUO1

• In a gel retardation assay poly(dG-m5dC) in the Z-form strongly diminishes the binding of tRNA to zuotin [10].
• Analysis of published DNA microarray experiments reveals conditions under which Ssb1, Ssb2, Ssz1, and Zuo1 transcript levels are regulated independently of those of genes encoding ribosomal proteins [11].

References