High impact information on TEF4

• We identified SAL6, TEF4, and YDL219w, all of which likely affect nonsense suppression via alteration of the protein synthesis machinery [1].
• The cold-sensitive phenotype of drs2 is suppressed by extra copies of the TEF3 gene, which encodes a yeast homolog of eukaryotic translation elongation factor EF-1 gamma [2].
• However, the second form of eEF1Bgamma encoded by the TEF4 gene contains serine 11, which may act catalytically [3].
• Ten cDNA clones were identified as transcriptional enhancer factor-4 (TEF-4) [4].
• When COS-7 cells were transfected with TEF-4, CCTalpha promoter-luciferase reporter activity and CCTalpha mRNA levels increased [4].

Biological context of TEF4

• These results provide the first evidence that TEF-4 is an important regulator of CCTalpha gene expression [4].

Other interactions of TEF4

• Unlike TEF3, extra copies of TEF4 do not suppress the cold-sensitive 40S ribosomal subunit deficiency of a drs2 strain [5].

Analytical, diagnostic and therapeutic context of TEF4

• Gel-shift analysis using radiolabeled Eb fragment as a probe showed that cell extracts from yeast expressing hemagglutinin-tagged TEF-4 caused a marked band retardation that could be prevented with an anti-hemagglutinin antibody [4].

References