High impact information on LOC85391

• In the spliceosomal C1 complex, snoRNP proteins efficiently assemble onto snoRNA sequences if they are located about 50 nt upstream of the intron branchpoint [1].
• In the September 1 issue of Molecular Cell, demonstrate that a spliceosomal intron binding protein, IBP160, couples box C/D snoRNA processing with pre-mRNA splicing in the C1 splicing complex [2].
• Chromatin immunoprecipitation experiments show that polyadenylation factors and Rat1 localize to snoRNA genes, but mutations that disrupt poly(A) site cleavage or Rat1 activity do not lead to termination defects at these genes [3].
• These data suggest that trypanosomatids do not obey the general 5-bp rule for snoRNA-mediated methylation [4].
• Prior to this study, only 28 snoRNA genes had been identified from Caenorhabditis elegans, indicating that most snoRNA genes are hidden in the worm genome, which represents a simple multicellular metazoan [5].

Biological context of LOC85391

• In this study, a genome-wide analysis of the two major families of snoRNA genes in C. elegans was performed using the snoscan and snoGPS programs incorporating comparative genome analyses [5].
• Notably, 6 box C/D snoRNA genes were located in the antisense orientation of introns [5].
• snoSeeker: an advanced computational package for screening of guide and orphan snoRNA genes in the human genome [6].

Analytical, diagnostic and therapeutic context of LOC85391

• Therefore, in this work, we set up two PCR reactions, TcH2AF/R and TrFR2, to distinguish T. cruzi from T. rangeli in mixed infections of vectors based on amplification of the histone H2A/SIRE and the small nucleolar RNA Cl1 genes, respectively [7].

References