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Gene Review

RPL25  -  ribosomal 60S subunit protein L25

Saccharomyces cerevisiae S288c

Synonyms: 60S ribosomal protein L25, RP16L, YL25, YOL127W, YP42'
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High impact information on RPL25

  • Using a visual assay for export of the 60S ribosomal subunit, we isolated a ts-lethal mutation, rix9-1, which causes nucleolar accumulation of an Rpl25p-eGFP reporter construct [1].
  • However, deletion of ARX1 or the fusion of enhanced GFP (eGFP) to Rpl25 suppressed the cold sensitivity of an rei1Delta mutant [2].
  • Depletion of Nop7p leads to nuclear accumulation of pre-60S particles, indicating a defect in subunit export, and it interacts genetically with a tagged form of the ribosomal protein Rpl25p, consistent with a role in subunit assembly [3].
  • However, under conditions of stress, PGK1 and RPL25 transcripts accumulate in the nucleus whereas SSA4 RNA is exported [4].
  • Re-addition of nitrogen to cells starved for nitrogen in the presence of glucose failed to trigger activation of trehalase, caused strongly reduced and aberrant repression of CTT1 and SSA3, and failed to induce the upshift in RPL25 expression [5].

Biological context of RPL25

  • RNAs encoding Rpl25, Pgk1, and Ssa4 were examined for the effects of 3' UTRs, introns, RNA processing factors, nucleoporins, and transport factors on their export [4].

Physical interactions of RPL25

  • The presence of eGFP on Rpl25 or its neighboring protein Rpl35 reduced the binding of Arx1 to 60S subunits, suggesting that Arx1 binds to 60S subunits in the vicinity of the exit tunnel [2].

Other interactions of RPL25

  • Nitrogen induction of RPL1, RPL25 and RPS33 was much less dependent on the presence of the sugar, and only phosphorylated sugar caused a further increase in expression [6].


  1. Rlp7p is associated with 60S preribosomes, restricted to the granular component of the nucleolus, and required for pre-rRNA processing. Gadal, O., Strauss, D., Petfalski, E., Gleizes, P.E., Gas, N., Tollervey, D., Hurt, E. J. Cell Biol. (2002) [Pubmed]
  2. Nuclear Recycling of the Pre-60S Ribosomal Subunit-Associated Factor Arx1 Depends on Rei1 in Saccharomyces cerevisiae. Hung, N.J., Johnson, A.W. Mol. Cell. Biol. (2006) [Pubmed]
  3. Yeast Pescadillo is required for multiple activities during 60S ribosomal subunit synthesis. Oeffinger, M., Leung, A., Lamond, A., Tollervey, D., Lueng, A. RNA (2002) [Pubmed]
  4. Pre-mRNA processing factors are required for nuclear export. Brodsky, A.S., Silver, P.A. RNA (2000) [Pubmed]
  5. The Sch9 protein kinase in the yeast Saccharomyces cerevisiae controls cAPK activity and is required for nitrogen activation of the fermentable-growth-medium-induced (FGM) pathway. Crauwels, M., Donaton, M.C., Pernambuco, M.B., Winderickx, J., de Winde, J.H., Thevelein, J.M. Microbiology (Reading, Engl.) (1997) [Pubmed]
  6. Glucose-triggered signalling in Saccharomyces cerevisiae: different requirements for sugar phosphorylation between cells grown on glucose and those grown on non-fermentable carbon sources. Pernambuco, M.B., Winderickx, J., Crauwels, M., Griffioen, G., Mager, W.H., Thevelein, J.M. Microbiology (Reading, Engl.) (1996) [Pubmed]
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