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Gene Review

NOP1  -  rRNA methyltransferase NOP1

Saccharomyces cerevisiae S288c

Synonyms: D2870, Histone-glutamine methyltransferase, LOT3, Nucleolar protein 1, U3 small nucleolar RNA-associated protein NOP1, ...
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Disease relevance of NOP1

  • Human fibrillarin expressed in vitro migrates on SDS gels as a 36-kDa protein that is specifically immunoprecipitated by antisera from humans with scleroderma autoimmune disease [1].
  • We have isolated a 1.1-kilobase cDNA clone that encodes human fibrillarin by screening a hepatoma library in parallel with DNA probes derived from the fibrillarin genes of Saccharomyces cerevisiae (NOP1) and Xenopus laevis [1].
  • Saccharomyces cerevisiae mother cells undergo an aging program that includes morphologic changes, sterility, redistribution of the Sir transcriptional silencing complex from HM loci and telomeres to the nucleolus, alterations in nucleolar architecture, and accumulation of extrachromosomal ribosomal DNA circles (ERCs) [2].
  • In dysplastic adenomas and carcinomas, p27BBP displayed a large increase of its nucleolar component that was superimposable to argyrophylic nucleolar organizing region-associated proteins and was associated with the nuclear matrix [3].
  • Expression of nucleolar protein p120 in human lung cancer: difference in histological types as a marker for proliferation [4].

High impact information on NOP1


Biological context of NOP1


Anatomical context of NOP1


Associations of NOP1 with chemical compounds

  • snR31 is a RNA species of 225 nt. which has the trimethyl guanosine cap structure typical of small nuclear RNAs (snRNAs) and yeast small nucleolar RNAs (snoRNAs), and is associated with the nucleolar proteins fibrillarin (NOP1) and GAR1 [16].
  • Among the few proteins of the eukaryotic nucleolus that have been characterized, four proteins, nucleolin, fibrillarin, SSB1 and NSR1, possess a common structural motif, the GAR domain, which is rich in glycine and arginine residues [17].
  • The NOP77 allele which complements the synthetic lethal nop1 strains has an alanine at position 308, predicted to lie in helix alpha 1 of RRM3, whereas the non-complementing nop77-1 allele contains a proline at the corresponding position [18].
  • Growth on glucose medium results in the depletion of NOP1 over several generations, during which cell growth is progressively impaired [19].
  • We show that purified snoRNPs are able to reproduce the site-specific methylation pattern on target RNA and that the predicted S-adenosyl-L-methionine-binding region of Nop1p is responsible for the catalytic activity [20].

Physical interactions of NOP1

  • Our findings are consistent with the notion that nucleolar localization is a result of the binding interactions of various domains of NSR1 within the nucleolus rather than the presence of a specific nucleolar targeting signal [21].
  • We report the solution structure of Rnt1p dsRBD complexed to the 5' terminal hairpin of one of its small nucleolar RNA substrates, the snR47 precursor [22].
  • Several small nucleolar RNAs co-immunoprecipitate with Sen1 but differentially associate with the wild-type and mutant protein [23].
  • A novel ribonucleoprotein complex enriched in nucleolar proteins was purified from yeast extracts and constituents were identified by mass spectrometry [24].
  • The yeast nucleolar protein Cbf5p is involved in rRNA biosynthesis and interacts genetically with the RNA polymerase I transcription factor RRN3 [25].

Co-localisations of NOP1


Regulatory relationships of NOP1


Other interactions of NOP1

  • GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast [17].
  • We propose that NOP77p mediates specific interactions between NOP1p and the pre-rRNA [18].
  • Thus, SOF1 is a new component of the nucleolar rRNA processing machinery [9].
  • These results suggest that Nop5p functions with Nop1p in the execution of early pre-rRNA processing steps that lead to formation of 18 S rRNA [12].
  • The observation that three nucleolar proteins are modified by Hmt1p but are not exported from the nucleolus implies an alternate role for arginine methylation [30].

Analytical, diagnostic and therapeutic context of NOP1


  1. cDNA cloning and sequencing of human fibrillarin, a conserved nucleolar protein recognized by autoimmune antisera. Aris, J.P., Blobel, G. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  2. Passage through stationary phase advances replicative aging in Saccharomyces cerevisiae. Ashrafi, K., Sinclair, D., Gordon, J.I., Guarente, L. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  3. Expression of a highly conserved protein, p27BBP, during the progression of human colorectal cancer. Sanvito, F., Vivoli, F., Gambini, S., Santambrogio, G., Catena, M., Viale, E., Veglia, F., Donadini, A., Biffo, S., Marchisio, P.C. Cancer Res. (2000) [Pubmed]
  4. Expression of nucleolar protein p120 in human lung cancer: difference in histological types as a marker for proliferation. Uchiyama, B., Saijo, Y., Kumano, N., Abe, T., Fujimura, S., Ohkuda, K., Handa, M., Satoh, K., Nukiwa, T. Clin. Cancer Res. (1997) [Pubmed]
  5. The Shwachman-Bodian-Diamond syndrome protein mediates translational activation of ribosomes in yeast. Menne, T.F., Goyenechea, B., Sánchez-Puig, N., Wong, C.C., Tonkin, L.M., Ancliff, P.J., Brost, R.L., Costanzo, M., Boone, C., Warren, A.J. Nat. Genet. (2007) [Pubmed]
  6. Yph1p, an ORC-interacting protein: potential links between cell proliferation control, DNA replication, and ribosome biogenesis. Du, Y.C., Stillman, B. Cell (2002) [Pubmed]
  7. Exit from mitosis is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex. Shou, W., Seol, J.H., Shevchenko, A., Baskerville, C., Moazed, D., Chen, Z.W., Jang, J., Shevchenko, A., Charbonneau, H., Deshaies, R.J. Cell (1999) [Pubmed]
  8. Net1, a Sir2-associated nucleolar protein required for rDNA silencing and nucleolar integrity. Straight, A.F., Shou, W., Dowd, G.J., Turck, C.W., Deshaies, R.J., Johnson, A.D., Moazed, D. Cell (1999) [Pubmed]
  9. A U3 snoRNP protein with homology to splicing factor PRP4 and G beta domains is required for ribosomal RNA processing. Jansen, R., Tollervey, D., Hurt, E.C. EMBO J. (1993) [Pubmed]
  10. Isolation and sequencing of NOP1. A yeast gene encoding a nucleolar protein homologous to a human autoimmune antigen. Henríquez, R., Blobel, G., Aris, J.P. J. Biol. Chem. (1990) [Pubmed]
  11. A small nucleolar RNP protein is required for pseudouridylation of eukaryotic ribosomal RNAs. Bousquet-Antonelli, C., Henry, Y., G'elugne, J.P., Caizergues-Ferrer, M., Kiss, T. EMBO J. (1997) [Pubmed]
  12. Nop5p is a small nucleolar ribonucleoprotein component required for pre-18 S rRNA processing in yeast. Wu, P., Brockenbrough, J.S., Metcalfe, A.C., Chen, S., Aris, J.P. J. Biol. Chem. (1998) [Pubmed]
  13. An unusual fibrillarin gene and protein: structure and functional implications. David, E., McNeil, J.B., Basile, V., Pearlman, R.E. Mol. Biol. Cell (1997) [Pubmed]
  14. The organization of ribosomal RNA processing correlates with the distribution of nucleolar snRNAs. Beven, A.F., Lee, R., Razaz, M., Leader, D.J., Brown, J.W., Shaw, P.J. J. Cell. Sci. (1996) [Pubmed]
  15. Genome-wide nuclear morphology screen identifies novel genes involved in nuclear architecture and gene-silencing in Saccharomyces cerevisiae. Teixeira, M.T., Dujon, B., Fabre, E. J. Mol. Biol. (2002) [Pubmed]
  16. Identification and functional analysis of a novel yeast small nucleolar RNA. Dandekar, T., Tollervey, D. Nucleic Acids Res. (1993) [Pubmed]
  17. GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast. Girard, J.P., Lehtonen, H., Caizergues-Ferrer, M., Amalric, F., Tollervey, D., Lapeyre, B. EMBO J. (1992) [Pubmed]
  18. Synthetic lethality with fibrillarin identifies NOP77p, a nucleolar protein required for pre-rRNA processing and modification. Bergès, T., Petfalski, E., Tollervey, D., Hurt, E.C. EMBO J. (1994) [Pubmed]
  19. The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast. Tollervey, D., Lehtonen, H., Carmo-Fonseca, M., Hurt, E.C. EMBO J. (1991) [Pubmed]
  20. Purified box C/D snoRNPs are able to reproduce site-specific 2'-O-methylation of target RNA in vitro. Galardi, S., Fatica, A., Bachi, A., Scaloni, A., Presutti, C., Bozzoni, I. Mol. Cell. Biol. (2002) [Pubmed]
  21. Multiple regions of NSR1 are sufficient for accumulation of a fusion protein within the nucleolus. Yan, C., Mélèse, T. J. Cell Biol. (1993) [Pubmed]
  22. Structural basis for recognition of the AGNN tetraloop RNA fold by the double-stranded RNA-binding domain of Rnt1p RNase III. Wu, H., Henras, A., Chanfreau, G., Feigon, J. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  23. The yeast SEN1 gene is required for the processing of diverse RNA classes. Ursic, D., Himmel, K.L., Gurley, K.A., Webb, F., Culbertson, M.R. Nucleic Acids Res. (1997) [Pubmed]
  24. Association of yeast RNA polymerase I with a nucleolar substructure active in rRNA synthesis and processing. Fath, S., Milkereit, P., Podtelejnikov, A.V., Bischler, N., Schultz, P., Bier, M., Mann, M., Tschochner, H. J. Cell Biol. (2000) [Pubmed]
  25. The yeast nucleolar protein Cbf5p is involved in rRNA biosynthesis and interacts genetically with the RNA polymerase I transcription factor RRN3. Cadwell, C., Yoon, H.J., Zebarjadian, Y., Carbon, J. Mol. Cell. Biol. (1997) [Pubmed]
  26. Mrd1p is required for processing of pre-rRNA and for maintenance of steady-state levels of 40 S ribosomal subunits in yeast. Jin, S.B., Zhao, J., Bjork, P., Schmekel, K., Ljungdahl, P.O., Wieslander, L. J. Biol. Chem. (2002) [Pubmed]
  27. Net1 stimulates RNA polymerase I transcription and regulates nucleolar structure independently of controlling mitotic exit. Shou, W., Sakamoto, K.M., Keener, J., Morimoto, K.W., Traverso, E.E., Azzam, R., Hoppe, G.J., Feldman, R.M., DeModena, J., Moazed, D., Charbonneau, H., Nomura, M., Deshaies, R.J. Mol. Cell (2001) [Pubmed]
  28. Role of Histone Deacetylase Rpd3 in Regulating rRNA Gene Transcription and Nucleolar Structure in Yeast. Oakes, M.L., Siddiqi, I., French, S.L., Vu, L., Sato, M., Aris, J.P., Beyer, A.L., Nomura, M. Mol. Cell. Biol. (2006) [Pubmed]
  29. Distinct nuclear and cytoplasmic functions of the S. pombe Cdc14-like phosphatase Clp1p/Flp1p and a role for nuclear shuttling in its regulation. Trautmann, S., McCollum, D. Curr. Biol. (2005) [Pubmed]
  30. In vivo analysis of nucleolar proteins modified by the yeast arginine methyltransferase Hmt1/Rmt1p. Xu, C., Henry, P.A., Setya, A., Henry, M.F. RNA (2003) [Pubmed]
  31. SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is associated with the snR10 and snR11 small nuclear RNAs. Clark, M.W., Yip, M.L., Campbell, J., Abelson, J. J. Cell Biol. (1990) [Pubmed]
  32. A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability. Schimmang, T., Tollervey, D., Kern, H., Frank, R., Hurt, E.C. EMBO J. (1989) [Pubmed]
  33. U24, a novel intron-encoded small nucleolar RNA with two 12 nt long, phylogenetically conserved complementarities to 28S rRNA. Qu, L.H., Henry, Y., Nicoloso, M., Michot, B., Azum, M.C., Renalier, M.H., Caizergues-Ferrer, M., Bachellerie, J.P. Nucleic Acids Res. (1995) [Pubmed]
  34. Molecular cloning of Aspergillus fumigatus CgrA, the ortholog of a conserved fungal nucleolar protein. Boettner, n.u.l.l., Huebner, N., Rhodes, J.C., Askew, D.S. Med. Mycol. (2001) [Pubmed]
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