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Gene Review

RPL5  -  ribosomal 60S subunit protein L5

Saccharomyces cerevisiae S288c

Synonyms: 60S ribosomal protein L5, L1, L1a, LPI14, LPI14W, ...
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Disease relevance of RPL5

  • H. cutirubrum HL19, however, has structural identity with E. coli L5 and seems to be related to yeast YL3 and, hence, may be an evolutionary link between the prokaryotic and eukaryotic 5 S rRNA-binding proteins [1].
  • A novel operon organization involving the genes for chorismate synthase (aromatic biosynthesis pathway) and ribosomal GTPase center proteins (L11, L1, L10, L12: rplKAJL) in cyanobacterium Synechocystis PCC 6803 [2].
  • The quadrivalent HPV vaccine is a mixture of four HPV type-specific VLPs prepared from the L1 proteins of HPV 6, 11, 16, and 18 combined with an aluminum adjuvant [3].

High impact information on RPL5

  • A conditional null mutant in which the only copy of RPL1 is under control of the repressible GAL1 promoter was constructed [4].
  • Ribosomal protein L1 from Saccharomyces cerevisiae binds 5S rRNA and can be released from intact 60S ribosomal subunits as an L1-5S ribonucleoprotein (RNP) particle [4].
  • The 5 S RNA binding protein (YL3) also appears not to influence directly the maturation process, but rather to play a role in protecting the rRNA from further degradation by "housekeeping" nucleases [5].
  • To identify residues important for binding of Saccharomyces cerevisiae rpL1 to 5 S rRNA and assembly into functional ribosomes, we have isolated mutant alleles of the yeast RPL1 gene by site-directed and random mutagenesis [6].
  • UPF1-3EP and L1 co-distributed with polyribosomes fractionated in a 7-47% sucrose gradient [7].

Biological context of RPL5


Anatomical context of RPL5

  • Multiple regions of yeast ribosomal protein L1 are important for its interaction with 5 S rRNA and assembly into ribosomes [6].
  • Immunodepletion of the cell-free system with anti-YL3 IgG was found to increase the sensitivity of 5 S rRNA synthesis to inhibition by 5 S rRNA [11].
  • When polyribosomes were disaggregated, UPF1-3EP and L1 accumulated in fractions coincident with 80S ribosomal particles [7].

Associations of RPL5 with chemical compounds


Physical interactions of RPL5

  • A two-dimensional gel electrophoresis method was used to demonstrate assembly of newly synthesized 5 S rRNA into a ribonucleoprotein complex containing ribosomal protein YL3 [11].
  • Contributions of the highly conserved K270 and its neighboring K271 in the C-terminal region of the yeast ribosomal protein L1 to 5S rRNA binding and ribosome assembly were examined by in vivo and in vitro studies on the consequences of 14 substitution mutations [14].

Other interactions of RPL5

  • An L1-5S RNP particle not associated with ribosomal particles was detected by coimmunoprecipitation of L1 and 5S rRNA [4].
  • We propose a model for the regulation of yeast 5 S rRNA synthesis in which YL3 competes with the presumptive transcription factor IIIA analog for binding of 5 S rRNA [11].

Analytical, diagnostic and therapeutic context of RPL5


  1. The primary structure of rat ribosomal protein L5. A comparison of the sequence of amino acids in the proteins that interact with 5 S rRNA. Chan, Y.L., Lin, A., McNally, J., Wool, I.G. J. Biol. Chem. (1987) [Pubmed]
  2. A novel operon organization involving the genes for chorismate synthase (aromatic biosynthesis pathway) and ribosomal GTPase center proteins (L11, L1, L10, L12: rplKAJL) in cyanobacterium Synechocystis PCC 6803. Schmidt, J., Bubunenko, M., Subramanian, A.R. J. Biol. Chem. (1993) [Pubmed]
  3. Quadrivalent Human Papillomavirus Vaccine: Recommendations of the Advisory Committee on Immunization Practices (ACIP). Markowitz, L.E., Dunne, E.F., Saraiya, M., Lawson, H.W., Chesson, H., Unger, E.R. MMWR. Recommendations and reports : Morbidity and mortality weekly report. Recommendations and reports / Centers for Disease Control (2007) [Pubmed]
  4. Yeast ribosomal protein L1 is required for the stability of newly synthesized 5S rRNA and the assembly of 60S ribosomal subunits. Deshmukh, M., Tsay, Y.F., Paulovich, A.G., Woolford, J.L. Mol. Cell. Biol. (1993) [Pubmed]
  5. Ribosomal 5 S rRNA maturation in Saccharomyces cerevisiae. Lee, Y., Nazar, R.N. J. Biol. Chem. (1997) [Pubmed]
  6. Multiple regions of yeast ribosomal protein L1 are important for its interaction with 5 S rRNA and assembly into ribosomes. Deshmukh, M., Stark, J., Yeh, L.C., Lee, J.C., Woolford, J.L. J. Biol. Chem. (1995) [Pubmed]
  7. The majority of yeast UPF1 co-localizes with polyribosomes in the cytoplasm. Atkin, A.L., Altamura, N., Leeds, P., Culbertson, M.R. Mol. Biol. Cell (1995) [Pubmed]
  8. Unbalanced regulation of the ribosomal 5 S RNA-binding protein in Saccharomyces cerevisiae expressing mutant 5 S rRNAs. Tang, B., Nazar, R.N. J. Biol. Chem. (1992) [Pubmed]
  9. A rice (Oryza sativa L.) cDNA encodes a protein sequence homologous to the eukaryotic ribosomal 5S RNA-binding protein. Kim, J.K., Wu, R. Plant Mol. Biol. (1993) [Pubmed]
  10. Involvement of multiple basic amino acids in yeast ribosomal protein L1 in 5S rRNA recognition. Yeh, L.C., Lee, J.C. Nucleic Acids Symp. Ser. (1995) [Pubmed]
  11. Modulation of yeast 5 S rRNA synthesis in vitro by ribosomal protein YL3. A possible regulatory loop. Brow, D.A., Geiduschek, E.P. J. Biol. Chem. (1987) [Pubmed]
  12. Involvement of lysine and arginine residues in the binding of yeast ribosomal protein YL3 to 5S RNA. Vioque, A., Hernández, F., Palacián, E. Mol. Cell. Biochem. (1987) [Pubmed]
  13. The yeast 5S rRNA binding ribosomal protein YL3 is phosphorylated in vivo. Campos, F., Corona-Reyes, M., Zinker, S. Biochim. Biophys. Acta (1990) [Pubmed]
  14. Involvement of lysine 270 and lysine 271 of yeast 5S rRNA binding protein in RNA binding and ribosome assembly. Yeh, L.C., Deshmukh, M., Woolford, J.L., Lee, J.C. Biochim. Biophys. Acta (1996) [Pubmed]
  15. Electron microscopic visualisation of the 5S rRNA-YL3 complex from Saccharomyces cerevisiae. Kyle, K.M., Harauz, G. Mol. Cell. Biochem. (1992) [Pubmed]
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