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Gene Review:

SKI7 - Ski7p

Saccharomyces cerevisiae S288c

Synonyms: Superkiller protein 7, YOR076C, YOR29-27

Benard, L. et al., Inada, T. et al., Ridley, S.P. et al., Madrona, A.Y. et al., Mitchell, P. et al., et al.

Mitchell, Tollervey, Inada, Aiba,

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Disease relevance of SKI7

We mapped and cloned SKI7, a gene that negatively controls the copy number of L-A and M double-stranded RNA viruses in Saccharomyces cerevisiae [1].
Overexpression of Ski7p efficiently cured the satellite virus M2 due to a twofold-increased repression of non-poly(A) mRNA expression [1].

High impact information on SKI7

Degradation of the deadenylated mRNA required the Rrp4p and Ski7p components of the cytoplasmic exosome complex, as well as the putative RNA helicase Ski2p [2].
Furthermore, it was found that the 5' --> 3' decay pathway was accelerated for nonstop mRNA decay in the absence of Ski7p [3].
A novel mRNA surveillance for mRNA lacking a termination codon (nonstop mRNA) has been proposed in which Ski7p is thought to recognize stalled ribosomes at the 3' end of mRNA [3].
These data suggest that the activation of 3'-to-5' directed NMD is mediated through the interaction between Upf1p and the Ski7p N domain [4].
In an mktl host, L-A-HN double-stranded RNA excludes M2 double-stranded RNA at 30 degrees C but not at 20 degrees C. Recessive mutations suppressing the exclusion of M2 by L-A-HN in an mktl host include six ski (superkiller) genes, three of which (ski6, ski7 and ski8) are new genes [5].

Associations of SKI7 with chemical compounds

The ski7 mutant was hypersensitive to hygromycin B, a result also suggesting a role in translation [1].

Physical interactions of SKI7

Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p [6].

Other interactions of SKI7

It does so by forming a complex with two partner proteins, Ski2p and Ski3p, which complete a complex that is capable of recruiting and activating the exosome/Ski7p complex that functions in RNA degradation [7].

References

The ski7 antiviral protein is an EF1-alpha homolog that blocks expression of non-Poly(A) mRNA in Saccharomyces cerevisiae. Benard, L., Carroll, K., Valle, R.C., Masison, D.C., Wickner, R.B. J. Virol. (1999) [Pubmed]
An NMD pathway in yeast involving accelerated deadenylation and exosome-mediated 3'-->5' degradation. Mitchell, P., Tollervey, D. Mol. Cell (2003) [Pubmed]
Translation of aberrant mRNAs lacking a termination codon or with a shortened 3'-UTR is repressed after initiation in yeast. Inada, T., Aiba, H. EMBO J. (2005) [Pubmed]
Interaction between Ski7p and Upf1p is required for nonsense-mediated 3'-to-5' mRNA decay in yeast. Takahashi, S., Araki, Y., Sakuno, T., Katada, T. EMBO J. (2003) [Pubmed]
Superkiller mutations in Saccharomyces cerevisiae suppress exclusion of M2 double-stranded RNA by L-A-HN and confer cold sensitivity in the presence of M and L-A-HN. Ridley, S.P., Sommer, S.S., Wickner, R.B. Mol. Cell. Biol. (1984) [Pubmed]
Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p. Wang, L., Lewis, M.S., Johnson, A.W. RNA (2005) [Pubmed]
The structure of Ski8p, a protein regulating mRNA degradation: Implications for WD protein structure. Madrona, A.Y., Wilson, D.K. Protein Sci. (2004) [Pubmed]

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