High impact information on MDM1

• The Saccharomyces cerevisiae protein MDM1 displays structure and solubility properties that are similar to those of intermediate filament proteins of animal cells [1].
• These structures disappear and organelle inheritance is disrupted when mdm1 mutant cells are incubated at the restrictive temperature [2].
• Mutational analysis of Mdm1p function in nuclear and mitochondrial inheritance [2].
• Genetic crosses of yeast strains containing different mdm1 alleles revealed complex genetic interactions including intragenic suppression, synthetic phenotypes, and intragenic complementation [2].
• Nuclear and mitochondrial inheritance in yeast depends on novel cytoplasmic structures defined by the MDM1 protein [3].

Biological context of MDM1

• The MDM1 gene was cloned by complementation, and its sequence revealed an open reading frame encoding a potential protein product of 51.5 kD [3].
• The mdm1 lesion also retards nuclear division and prevents the transfer of nuclei into the buds [4].
• The mdm1 and mdm2 mutations are cell-cycle specific, displaying an execution point in late G1 or early S phase [4].

Anatomical context of MDM1

• These results suggest that novel cytoplasmic structures containing the MDM1 protein mediate organelle inheritance in yeast [3].
• Neither mdm1 or mdm2 affects the transfer of vacuolar material into the buds or causes apparent changes in the tubulin- or actin-based cytoskeletons [4].

Analytical, diagnostic and therapeutic context of MDM1

• Antibodies against the MDM1 protein recognized a 51-kD polypeptide that was localized by indirect immunofluorescence to a novel pattern of spots and punctate arrays distributed throughout the yeast cell cytoplasm [3].

References