High impact information on CDK10

• We find that Cdk10 binds full length Ets2 in vitro and in vivo and inhibits Ets2 transactivation in mammalian cells [1].
• Cdk10 is a Cdc2-related kinase that may play a role in regulating the G2/M phase of the cell cycle [1].
• Comparison of the amino acid sequences of murine and human cdk10 shows high homology [2].
• CDK10 mRNA quantity was not found to be correlated with cell proliferation status in HeLa or WI38 cell cultures or in human tissues [3].
• RESULTS: Some of the differentially expressed genes complied with expected gene expression including upregulation of c-jun and CDK10 [4].

Associations of CDK10 with chemical compounds

• Patients with ER alpha-positive tumors that express low levels of CDK10 relapse early on tamoxifen, demonstrating the clinical significance of these observations [5].

Biological context of CDK10

• We report in this study that Cdk10 interacts with the N-terminus of the Ets2 transcription factor, which contains the highly conserved Pointed domain and transactivation domain [1].
• Identification of murine cdk10: Association with Ets2 transcription factor and effects on the cell cycle [2].
• A role at the G2/M transition has been suggested for an isoform of cdk10, while the alternative splicing form interacts with the N-terminus of the Ets2 transcription factor [2].

Anatomical context of CDK10

• Murine cdk10 binds Ets2 transcription factors in vitro, does not show a direct involvement in the G2/M transition and, therefore, does not affect the proliferation rate of the cell lines analyzed [2].

Physical interactions of CDK10

• The Pointed domain has been implicated in protein-protein interactions and we find that Ets2 requires an intact Pointed domain to bind Cdk10 [1].

Analytical, diagnostic and therapeutic context of CDK10

• Relative levels of the four CDK10 isoforms were studied by RT-PCR, of which three were principally expressed [3].

References