High impact information on MLH3

• Meiotic arrest and aneuploidy in MLH3-deficient mice [1].
• Taken together, these data imply modulation of a basic Mlh1 function via combination with the three other MutL homologs and suggest specifically that Mlh1 combines with Mlh3 to promote meiotic crossing-over [2].
• Conversely, crossing-over in the mlh3 mutant is reduced to approximately 70% of wild-type levels but correction of heteroduplex is normal [2].
• The Saccharomyces cerevisiae MLH3 gene functions in MSH3-dependent suppression of frameshift mutations [3].
• Combination of mutations in MLH3 and MSH6 caused a synergistic increase in the hom3-10 reversion rate, whereas the hom3-10 reversion rate in an mlh3 msh3 double mutant was the same as in the respective single mutants [3].

Biological context of MLH3

• In this report, we show that yeast Mlh3 co-immunoprecipitates with Sgs1 helicase in sporulating cells at late stage of meiotic prophase I [4].

Physical interactions of MLH3

• MLH3 interacted with MLH1 in a two-hybrid system [3].

Other interactions of MLH3

• This analysis demonstrates, for the first time, that yeast Mlh2p plays a role in the repair of mutational intermediates, and extends earlier results implicating Mlh3p in mismatch repair [5].
• Similar results were observed when the accumulation of mutations at frameshift hot spots in the LYS2 gene was analyzed, although mutation of MLH3 did not cause the same extent of affect at every LYS2 frameshift hot spot [3].

References