High impact information on CCIN

• We have studied the association of boar calicin with F-actin and the distribution of boar and human calicin during spermiogenesis compared with the distribution of actin [1].
• Taken together, the results suggest that the affinity of calicin to F-actin allows targeting of calicin at the subacrosomal space of round spermatids, and that its ability to form homomultimers contributes to the formation of a rigid calyx [1].
• Calicin was purified from boar sperm heads under nondenaturating conditions [1].
• Presence and localization of the 60 KD calicin in human spermatozoa presenting postacrosomal sheath defects: preliminary results [2].
• In the head, a dense perinuclear layer is made of basic proteins (calicin, cylicin, etc) associated with calmodulin and actin remnants [3].

Biological context of CCIN

• Actin-binding properties and colocalization with actin during spermiogenesis of mammalian sperm calicin [1].

Anatomical context of CCIN

• According to immunocytochemical results, calicin is present (together with actin) in the acrosomal region of round spermatids and is mainly localized in the postacrosomal region of late spermatids and spermatozoa [1].
• The fate of human sperm calicin in the zona-free hamster egg fertilized in vitro has been analysed at the light and electron microscope level using polyclonal mouse anti-calicin antibodies [4].

Analytical, diagnostic and therapeutic context of CCIN

• Gel filtration studies showed that calicin forms homomultimers (tetramers and higher polymers) [1].

References