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Gene Review

mpt64  -  immunogenic protein Mpt64

Mycobacterium tuberculosis H37Rv

 
 
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Disease relevance of mpt64

  • A comparison showed mpt64 and the gene encoding MPB64 from Mycobacterium bovis BCG Tokyo to be identical except for one silent mutation [1].
  • In this double-blind study, 61 samples, consisting of endometrial aspirates (EAs), endometrial biopsies (EBs) and fluid from the pouch of Douglas (POD), from 25 women suffering from infertility were investigated for the presence of the mpt64 gene of Mycobacterium tuberculosis by PCR and correlated with laparoscopic findings [2].
  • Multiple sampling from different sites and amplification of the mpt64 gene segment by PCR offered increased sensitivity in determining tuberculous aetiology in female infertility [2].
 

High impact information on mpt64

  • The addition of a eukaryotic leader sequence to mpt64 did not significantly increase the T-cell or antibody response [3].
  • Southern blot analyses revealed differences in the restriction enzyme patterns within the M. tuberculosis substrains as well as within the M. bovis BCG substrains, indicating either different chromosomal localization of mpt64 or that mutations have occurred at different locations on the chromosomes [1].
  • RD2, a 10.7-kb DNA segment containing a novel repetitive element and the previously identified mpt-64 gene, was conserved in all virulent laboratory and clinical tubercle bacilli tested and was deleted only from substrains derived from the original BCG Pasteur strain after 1925 [4].
 

Analytical, diagnostic and therapeutic context of mpt64

  • Screening by PCR of 56 clinical isolates of M. tuberculosis from Danish and Tanzanian patients demonstrated the presence of mpt64 in all of the strains [5].

References

  1. Cloning and B-cell-epitope mapping of MPT64 from Mycobacterium tuberculosis H37Rv. Oettinger, T., Andersen, A.B. Infect. Immun. (1994) [Pubmed]
  2. Improved diagnostic value of PCR in the diagnosis of female genital tuberculosis leading to infertility. Bhanu, N.V., Singh, U.B., Chakraborty, M., Suresh, N., Arora, J., Rana, T., Takkar, D., Seth, P. J. Med. Microbiol. (2005) [Pubmed]
  3. Differential protective efficacy of DNA vaccines expressing secreted proteins of Mycobacterium tuberculosis. Kamath, A.T., Feng, C.G., Macdonald, M., Briscoe, H., Britton, W.J. Infect. Immun. (1999) [Pubmed]
  4. Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M. bovis. Mahairas, G.G., Sabo, P.J., Hickey, M.J., Singh, D.C., Stover, C.K. J. Bacteriol. (1996) [Pubmed]
  5. Mapping of the delayed-type hypersensitivity-inducing epitope of secreted protein MPT64 from Mycobacterium tuberculosis. Oettinger, T., Holm, A., Mtoni, I.M., Andersen, A.B., Hasløov, K. Infect. Immun. (1995) [Pubmed]
 
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