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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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RNF8  -  ring finger protein 8, E3 ubiquitin...

Homo sapiens

Synonyms: E3 ubiquitin-protein ligase RNF8, KIAA0646, RING finger protein 8, hRNF8
 
 
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High impact information on RNF8

  • In COS7 cells, overexpressed RNF8 colocalized and interacted with RXR alpha in the nucleus, as shown by fluorescence resonance energy transfer [1].
  • The RING finger protein RNF8 recruits UBC13 for lysine 63-based self polyubiquitylation [2].
  • Transfection of COS-7 cells with a green fluorescent protein chimera showed that RNF8 was localized in the nucleus, and ARA54 in both the cytoplasm and nucleus [3].
  • Ubiquitination of wild-type ARA54 and RNF8, expressed in COS-7 cells, was also observed, and a proteasome inhibitor, MG132, prevented the degradation of these wild-type proteins, but was much less effective in protecting the RING mutants [3].
 

Biological context of RNF8

  • Using yeast two-hybrid screening, beta-galactosidase assays, and pull-down assays, we show that RNF8, a RING finger protein recently isolated as a protein binding to a ubiquitin-conjugating enzyme, binds to RXR alpha through the N-terminal regions of both proteins [1].
 

Other interactions of RNF8

  • The RING finger protein, RNF8, interacts with retinoid X receptor alpha and enhances its transcription-stimulating activity [1].
  • These data suggest that MDC1-mediated and RNF8-executed histone ubiquitylation protects genome integrity by licensing the DSB-flanking chromatin to concentrate repair factors near the DNA lesions [4].

References

  1. The RING finger protein, RNF8, interacts with retinoid X receptor alpha and enhances its transcription-stimulating activity. Takano, Y., Adachi, S., Okuno, M., Muto, Y., Yoshioka, T., Matsushima-Nishiwaki, R., Tsurumi, H., Ito, K., Friedman, S.L., Moriwaki, H., Kojima, S., Okano, Y. J. Biol. Chem. (2004) [Pubmed]
  2. The RING finger protein RNF8 recruits UBC13 for lysine 63-based self polyubiquitylation. Plans, V., Scheper, J., Soler, M., Loukili, N., Okano, Y., Thomson, T.M. J. Cell. Biochem. (2006) [Pubmed]
  3. N-Terminally extended human ubiquitin-conjugating enzymes (E2s) mediate the ubiquitination of RING-finger proteins, ARA54 and RNF8. Ito, K., Adachi, S., Iwakami, R., Yasuda, H., Muto, Y., Seki, N., Okano, Y. Eur. J. Biochem. (2001) [Pubmed]
  4. RNF8 ubiquitylates histones at DNA double-strand breaks and promotes assembly of repair proteins. Mailand, N., Bekker-Jensen, S., Faustrup, H., Melander, F., Bartek, J., Lukas, C., Lukas, J. Cell (2007) [Pubmed]
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