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Gene Review

UBE2M  -  ubiquitin-conjugating enzyme E2M

Homo sapiens

Synonyms: NEDD8 carrier protein, NEDD8 protein ligase, NEDD8-conjugating enzyme Ubc12, UBC-RS2, UBC12, ...
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High impact information on UBE2M

  • The structure and associated mutational analyses reveal molecular details of Ubc12 recruitment by NEDD8's E1 [1].
  • We show that expression of APP-BP1 in ts41 cells drives the cell cycle through the S-M checkpoint and that this function requires both hUba3 and hUbc12 [2].
  • To characterize this family of E3 ligases in intact cells, we generated a cell line with tetracycline-inducible expression of a dominant-negative mutant of the Nedd8-conjugating enzyme Ubc12, a reported inhibitor of cullin neddylation [3].
  • Using this cell line, we demonstrate that the substrate recognition subunit Skp2 and the adaptor protein Skp1 are subject to Ubc12-dependent autoubiquitination and degradation [3].
  • In vitro systems for NEDD8 conjugation by Ubc12 [4].

Other interactions of UBE2M

  • One of the identified targets of copine I is the NEDD8-conjugating enzyme UBC12 (ubiquitin C12), that promotes the degradation of IkappaB through the ubiquitin ligase enzyme complex SCF(betaTrCP) [5].
  • Therefore the copine dominant-negative construct might inhibit TNF-alpha signalling by dysregulation or mislocalization of UBC12 [5].


  1. Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1. Huang, D.T., Paydar, A., Zhuang, M., Waddell, M.B., Holton, J.M., Schulman, B.A. Mol. Cell (2005) [Pubmed]
  2. The amyloid precursor protein-binding protein APP-BP1 drives the cell cycle through the S-M checkpoint and causes apoptosis in neurons. Chen, Y., McPhie, D.L., Hirschberg, J., Neve, R.L. J. Biol. Chem. (2000) [Pubmed]
  3. Characterization of cullin-based E3 ubiquitin ligases in intact mammalian cells - Evidence for cullin dimerization. Chew, E.H., Poobalasingam, T., Hawkey, C.J., Hagen, T. Cell. Signal. (2007) [Pubmed]
  4. In vitro systems for NEDD8 conjugation by Ubc12. Chiba, T. Meth. Enzymol. (2005) [Pubmed]
  5. Calcium-dependent regulation of tumour necrosis factor-alpha receptor signalling by copine. Tomsig, J.L., Sohma, H., Creutz, C.E. Biochem. J. (2004) [Pubmed]
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