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Gene Review:

dnaA - chromosomal replication initiation protein

Escherichia coli O157:H7 str. Sakai

Riber, L. et al., Wilkinson, T.G. et al.

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Disease relevance of dnaA

Hda-mediated inactivation of the DnaA protein and dnaA gene autoregulation act in concert to ensure homeostatic maintenance of the Escherichia coli chromosome [1].

High impact information on dnaA

Based on these observations, we propose that both RIDA and dnaA gene autoregulation are required as homeostatic mechanisms to ensure that initiation of replication occurs at the same time relative to cell mass in each cell cycle [1].
The cellular level of Hda correlated directly with dnaA gene expression such that Hda deficiency led to reduced dnaA gene expression, and overproduction of Hda led to DnaA overproduction [1].
To examine whether methylation of the GATC sites present in the dnaA promoter region is responsible for the strict temporal coordination of initiation events at oriC as measured by the synchrony of initiation, we introduced point mutations eliminating three (TGW1) and five (TGW2) of the six GATC sites present in the dnaA promoter region [2].
The synchrony phenotype persists after elimination of multiple GATC sites from the dnaA promoter of Escherichia coli [2].

References

Hda-mediated inactivation of the DnaA protein and dnaA gene autoregulation act in concert to ensure homeostatic maintenance of the Escherichia coli chromosome. Riber, L., Olsson, J.A., Jensen, R.B., Skovgaard, O., Dasgupta, S., Marinus, M.G., Løbner-Olesen, A. Genes Dev. (2006) [Pubmed]
The synchrony phenotype persists after elimination of multiple GATC sites from the dnaA promoter of Escherichia coli. Wilkinson, T.G., Kedar, G.C., Lee, C., Guzmán, E.C., Smith, D.W., Zyskind, J.W. J. Bacteriol. (2006) [Pubmed]

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