Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Gene Review:

ECs5322 - primosomal protein DnaI

Escherichia coli O157:H7 str. Sakai

Soultanas, P., Ng, J.Y. et al., Turner, I.J. et al., Masai, H. et al., Liu, J.H. et al.

Soultanas,

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Disease relevance of ECs5322

The Escherichia coli gene encoding one of the primosomal proteins, protein i, was cloned by the use of synthetic oligonucleotide probes [1].
The Bacillus subtilis DnaD protein is an essential protein and a component of the oriC and PriA primosomal cascades, which are responsible for loading the main replicative ring helicase DnaC onto DNA [2].
In this paper we provide evidence for a functional interaction between the primosomal protein DnaI from B.subtilis and the main hexameric replicative helicase DnaB from Bacillus stearothermophilus [3].

High impact information on ECs5322

Crystal structure of PriB, a primosomal DNA replication protein of Escherichia coli [4].
However, the lack of sequence homology between the rest of the primosomal proteins indicates significant differences between these two bacterial species [3].

Analytical, diagnostic and therapeutic context of ECs5322

The presence and relative amounts of primosomal proteins in these complexes were determined by Western blotting [5].
Gel filtration chromatography was used to isolate both preprimosomal and primosomal complexes formed on single-stranded DNA-binding protein-coated phiX174 DNA by the combination of PriA, PriB, PriC, DnaT, DnaB, DnaC, and DnaG [5].

References

Cloning of the Escherichia coli gene for primosomal protein i: the relationship to dnaT, essential for chromosomal DNA replication. Masai, H., Bond, M.W., Arai, K. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
The Bacillus subtilis DnaD protein: a putative link between DNA remodeling and initiation of DNA replication. Turner, I.J., Scott, D.J., Allen, S., Roberts, C.J., Soultanas, P. FEBS Lett. (2004) [Pubmed]
A functional interaction between the putative primosomal protein DnaI and the main replicative DNA helicase DnaB in Bacillus. Soultanas, P. Nucleic Acids Res. (2002) [Pubmed]
Crystal structure of PriB, a primosomal DNA replication protein of Escherichia coli. Liu, J.H., Chang, T.W., Huang, C.Y., Chen, S.U., Wu, H.N., Chang, M.C., Hsiao, C.D. J. Biol. Chem. (2004) [Pubmed]
The ordered assembly of the phiX174-type primosome. II. Preservation of primosome composition from assembly through replication. Ng, J.Y., Marians, K.J. J. Biol. Chem. (1996) [Pubmed]

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