Disease relevance of ObPVgp2

• It consists of 6507 nucleotides and contains four open reading frames, exactly corresponding to the genomic organization of tobamoviruses known so far, i.e. encoding the 130K, 180K, 30K and coat proteins [1].

High impact information on ObPVgp2

• Three (two frame-shift and one non-sense) mutants with an intact 130K but a defective 180K protein gene were not infectious, while two mutants with a one-amino-acid insertion in the 180K protein gene were infectious [2].
• Taken together, these results indicate that it is likely that the ToMV-encoded suppressor, the 130K replication protein, blocks the utilization of silencing-associated small RNAs, so that a homology-dependent RNA degradation machinery is not newly formed [3].
• The four genes (130K, 180K, 30K and coat protein) could then be mapped at precise locations in the TMV genome [4].

Biological context of ObPVgp2

• Only three, which were in the common reading frame for both the 130K and 180K proteins, resulted in amino acid changes [5].
• Examination of the infection phenotypes of the 14 constructed chimeric viruses in BY showed that determinants defining the differential infection phenotype in BY reside in the 130K/180K replicase proteins and the 3' noncoding region [6].

Other interactions of ObPVgp2

• Remarkable differences between them were found in a part of the N-terminal portion of the 130K/180K protein and the C-terminal portion of the 30K protein [7].

References