Disease relevance of ObPVgp3

• It consists of 6507 nucleotides and contains four open reading frames, exactly corresponding to the genomic organization of tobamoviruses known so far, i.e. encoding the 130K, 180K, 30K and coat proteins [1].
• Identification of the function of the 30K protein was especially important because it was the first evidence that a plant virus possesses a cell-to-cell movement function [2].
• The nucleotide sequence of cloned cDNA copies of cowpea strain of tobacco mosaic virus RNA including the 30K protein cistron was determined [3].
• One hybrid virus, CAT-CP, with the insertion between the 30K and coat protein genes, replicated efficiently, produced an additional subgenomic RNA and CAT activity, and assembled into 350-nm virions, compared to 300-nm virions of wild-type TMV [4].
• To characterize common structural features of the 30K protein, the nucleotide sequence of the 30K protein gene of cucumber green mottle mosaic virus (CGMMV, a member of the tobamoviruses) RNA has been determined [5].

High impact information on ObPVgp3

• The sequence encompasses the 30K and the coat protein cistron which are located in residues 685-1, 479 and 203-682 from the 3' end of the genome respectively [6].
• Molecular cloning and nucleotide sequence of the 30K and the coat protein cistron of TMV (tomato strain) genome [6].
• The comparison of the deduced amino acid sequence between the tomato and the common strain shows that the 30K protein is composed of the conserved N-terminal four-fifth and the highly divergent region near the C-terminus [6].
• The four genes (130K, 180K, 30K and coat protein) could then be mapped at precise locations in the TMV genome [2].
• Complete nucleotide sequencing of the TMV-R genomic RNA revealed amino acid changes in the 130K/ 180K replicase proteins, the 30K protein, and the coat protein and nucleotide changes in the 5' and 3' noncoding regions compared to TMV-U1 [7].

Biological context of ObPVgp3

• The amino acid sequence homologies of the four encoded proteins (180K, 130K, 30K, coat protein) are from 95.9% to 98.0% compared with TMV-U1 [8].
• The 30K protein cistron was located at residue 676-1,527 from the 3' end of the genomic RNA [3].
• However, this mutant and a mutant that fused the 183K reading frame to the 30K reading frame did not replicate and move in intact plants [9].
• Single amino acid substitution in 30K protein of TMV defective in virus transport function [10].
• These data demonstrate consensus sequences that are optimal for other eukaryotic systems did not cause increased expression of the 30K gene in vivo [9].

Anatomical context of ObPVgp3

• Subcellular localization of the 30K protein in TMV-inoculated tobacco protoplasts [11].
• We formerly found that the 30K protein is localized to the plasmodesmata of TMV-infected plants [12].

Analytical, diagnostic and therapeutic context of ObPVgp3

• Tests with a 30K pore-size ultrafiltration membrane achieved 99% virus removal efficiency and a 5K membrane practically removed 100% of the virus in greenhouse wastewater [13].

References