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Gene Review:

purK - N5-carboxyaminoimidazole ribonucleotide...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0515, JW0511, purE2

Tiedeman, A.A. et al., Chen, Z.D. et al., Meyer, E. et al., Watanabe, W. et al., Sørensen, I.S. et al., et al.

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Disease relevance of purK

The avian enzyme lacks sequences corresponding to purK yet functions in E. coli [1].

High impact information on purK

In E. coli, AIR carboxylase is the product of genes purEK with the purK subunit postulated to have a role in CO2 binding [1].
However, the purT protein shows significant homology to the purK protein, also involved in purine biosynthesis [2].
Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway [3].

Chemical compound and disease context of purK

Identification and sequence analysis of Escherichia coli purE and purK genes encoding 5'-phosphoribosyl-5-amino-4-imidazole carboxylase for de novo purine biosynthesis [4].

Biological context of purK

These two genes formed an operon in which the termination codon of the purE gene overlapped the initiation codon of the purK gene [5].
In agreement with established complementation data, the catalytic subunit (deduced Mr, 17,782) was encoded by the purE gene, while the CO2-binding subunit (deduced Mr, 39,385) was encoded by the purK gene [5].
Two open reading frames are identified as homologs of the purE and purK purine biosynthetic genes in Escherichia coli [6].

References

Cloning of a chicken liver cDNA encoding 5-aminoimidazole ribonucleotide carboxylase and 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase by functional complementation of Escherichia coli pur mutants. Chen, Z.D., Dixon, J.E., Zalkin, H. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
Cloning and characterization of a new purine biosynthetic enzyme: a non-folate glycinamide ribonucleotide transformylase from E. coli. Marolewski, A., Smith, J.M., Benkovic, S.J. Biochemistry (1994) [Pubmed]
Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway. Meyer, E., Leonard, N.J., Bhat, B., Stubbe, J., Smith, J.M. Biochemistry (1992) [Pubmed]
Identification and sequence analysis of Escherichia coli purE and purK genes encoding 5'-phosphoribosyl-5-amino-4-imidazole carboxylase for de novo purine biosynthesis. Watanabe, W., Sampei, G., Aiba, A., Mizobuchi, K. J. Bacteriol. (1989) [Pubmed]
Nucleotide sequence analysis of the purEK operon encoding 5'-phosphoribosyl-5-aminoimidazole carboxylase of Escherichia coli K-12. Tiedeman, A.A., Keyhani, J., Kamholz, J., Daum, H.A., Gots, J.S., Smith, J.M. J. Bacteriol. (1989) [Pubmed]
Identification and sequence analysis of Sulfolobus solfataricus purE and purK genes. Sørensen, I.S., Dandanell, G. FEMS Microbiol. Lett. (1997) [Pubmed]

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