Disease relevance of purE

• It has been shown that the Escherichia coli purE locus specifying 5'-phosphoribosyl-5-amino-4-imidazole carboxylase in de novo purine nucleotide synthesis is divided into two cistrons [1].
• When the recombinant expression vector (pPfPRT2) was transformed and expressed in a Salmonella typhimurium mutant KP1684 (purE deoD hpt gpt), the active expressed protein complemented the hpt mutation in the bacteria [2].

High impact information on purE

• Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway [3].
• A highly conserved 16-nucleotide sequence, termed the PUR box, was found in the upstream region of purE by comparing the sequences of the purF and purMN operons [1].
• The purE mutations of CSH57A and DCSP22 were complemented by plasmids carrying ORF-18, while that of NK6051 was complemented by plasmids carrying ORF-39 [1].
• Two open reading frames are identified as homologs of the purE and purK purine biosynthetic genes in Escherichia coli [4].

Biological context of purE

• These two genes formed an operon in which the termination codon of the purE gene overlapped the initiation codon of the purK gene [5].
• In agreement with established complementation data, the catalytic subunit (deduced Mr, 17,782) was encoded by the purE gene, while the CO2-binding subunit (deduced Mr, 39,385) was encoded by the purK gene [5].

Analytical, diagnostic and therapeutic context of purE

• Identification and sequence analysis of Escherichia coli purE and purK genes encoding 5'-phosphoribosyl-5-amino-4-imidazole carboxylase for de novo purine biosynthesis [1].

References