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Gene Review

purC  -  phosphoribosylaminoimidazole...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK2472, JW2461, ade
 
 
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Disease relevance of purC

  • 5'-Phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide synthetase (EC 6.3.2.6), encoded by the purC gene of Escherichia coli K-12, catalyzes the synthesis of 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide from 5'-phosphoribosyl-5-aminoimidazole-4-carboxylic acid [1].
  • Identification of a purC gene from Streptococcus pneumoniae [2].
 

High impact information on purC

  • In common with other members of the purF family of amidotransferases, the recombinant enzyme possesses an NH2-terminal cysteine residue [3].
  • A PurR-hypoxanthine-DNA complex has been crystallized, with DNA encompassing the high affinity purF operator site and which is 16 base-pairs long with 5'-deoxynucleoside overhangs on each complementary strand [4].
  • We interpret our kinetic data as further evidence against ammonia-mediated nitrogen transfer from glutamine in the purF amidotransferase AS-B [5].
  • To facilitate the assay for CAIR production, the purC gene product, 5-aminoimidazole-4-N-succinylcarboxamide ribonucleotide (SAICAR) synthetase has also been overexpressed and purified to homogeneity [6].
  • A gene for a new lipoprotein in the dapA-purC interval of the Escherichia coli chromosome [7].
 

Biological context of purC

  • The DNA sequence was extended 1.281 kilobases upstream of the purC structural gene and overlapped with the previously determined dapA sequence [1].
  • The purC control region contains a binding site for and is regulated by the purine repressor, the product of the purR gene [1].
  • The putative transcription termination signals of the dapA-nlpB operon overlap the promoter of the adjacent purC gene [7].
  • In L. lactis there is a gene cluster, which contains five out of the 11 genes needed for the de novo biosynthesis of IMP, namely purC, orf, purQ, purL and purF [8].
 

Associations of purC with chemical compounds

  • PurE or purC strains [5'-phosphoribosyl-5-aminoimidazole (AIR)-accumulating] derepressed only 4-fold [9].
  • While such an observation has been made for a few members of the trpG amidotransferase family, our results appear to be the first demonstration that nitrogen transfer can occur from glutamine analogs in a purF amidotransferase [5].

References

  1. DNA sequence of the purC gene encoding 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide synthetase and organization of the dapA-purC region of Escherichia coli K-12. Tiedeman, A.A., DeMarini, D.J., Parker, J., Smith, J.M. J. Bacteriol. (1990) [Pubmed]
  2. Identification of a purC gene from Streptococcus pneumoniae. Hui, F.M., Morrison, D.A. J. Bacteriol. (1993) [Pubmed]
  3. Glutamine-dependent nitrogen transfer in Escherichia coli asparagine synthetase B. Searching for the catalytic triad. Boehlein, S.K., Richards, N.G., Schuster, S.M. J. Biol. Chem. (1994) [Pubmed]
  4. Crystallization and preliminary X-ray analysis of an Escherichia coli purine repressor-hypoxanthine-DNA complex. Schumacher, M.A., Choi, K.Y., Zalkin, H., Brennan, R.G. J. Mol. Biol. (1994) [Pubmed]
  5. Glutamic acid gamma-monohydroxamate and hydroxylamine are alternate substrates for Escherichia coli asparagine synthetase B. Boehlein, S.K., Schuster, S.M., Richards, N.G. Biochemistry (1996) [Pubmed]
  6. Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway. Meyer, E., Leonard, N.J., Bhat, B., Stubbe, J., Smith, J.M. Biochemistry (1992) [Pubmed]
  7. A gene for a new lipoprotein in the dapA-purC interval of the Escherichia coli chromosome. Bouvier, J., Pugsley, A.P., Stragier, P. J. Bacteriol. (1991) [Pubmed]
  8. Isolation and characterization of a purC(orf)QLF operon from Lactococcus [correction of Lactobacillus] lactis MG1614. Peltonen, T., Mäntsälä, P. Mol. Gen. Genet. (1999) [Pubmed]
  9. Dual control of the gua operon of Escherichia coli K12 by adenine and guanine nucleotides. Mehra, R.K., Drabble, W.T. J. Gen. Microbiol. (1981) [Pubmed]
 
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