Disease relevance of fabI

• Based on these data, it is concluded that EnvM is the NADH-dependent enoyl-ACP reductase (EC 1.3.1.9) of E. coli and we propose to rename the corresponding gene fabI [1].
• The envM gene from Salmonella typhimurium was cloned and sequenced [2].
• Conjugation and bacteriophage P1 transduction experiments in Escherichia coli showed that resistance to the antibacterial compound diazaborine is caused by an allelic form of the envM gene [2].
• FIP-gts, a fungal immunomodulatory protein found in Song-Shan Lingzhi (Ganodera tsugae) has been proposed to possess therapeutic effects on cancer and autoimmune diseases [3].

High impact information on fabI

• The putative bacterial enoyl-ACP reductase gene (envM) was identified on the basis of amino acid sequence similarities with the recently cloned plant enoyl-ACP reductase [4].
• Recombinant plasmids containing gene envM from a diazaborine-resistant S. typhimurium strain conferred the drug resistance phenotype to susceptible E. coli cells [2].
• A guanine-to-adenine exchange in the envM gene changing a Gly codon to a Ser codon was shown to be responsible for the resistance character [2].
• The phenotypes of temperature-sensitive qmeA and fabI mutants of Escherichia coli appear to be very similar [5].
• The nucleotide sequence of the Escherichia coli envM gene was determined [6].
• The G93V mutant form of FabI has been developed into a novel selectable marker for efficient molecular cloning and recombineering in E. coli [7].

Biological context of fabI

• Gene envM is preceded in E. coli by a 43-nucleotide-long structural element, termed 'box c', which occurs in several E. coli operons between structural genes [6].
• The plasmids carrying this DNA complemented a conditionally lethal envM mutant of E. coli [2].

References