Disease relevance of pspA

• The expression of specific PhoE mutant proteins leads to induction of the expression of the psp operon of Escherichia coli and the export of various plasmid-encoded precursors is retarded in a pspA mutant strain [1].
• Truncated forms of PspA that are secreted from Streptococcus pneumoniae and their use in functional studies and cloning of the pspA gene [2].
• Escherichia coli sigma54-dependent phage shock protein operon (pspA to -E) transcription is under the control of PspF, a constitutively active activator [3].

High impact information on pspA

• Precursor PhoE translocation was retarded in a pspB mutant strain to a similar extent as in a pspA mutant strain [1].
• PspF, the transcriptional activator of the pspA operon of Escherichia coli, which belongs to the enhancer binding protein (EBP) family of sigma54 activator proteins, is constitutively active in an in vitro transcription assay [4].
• As a result of this 13.1-kb deletion, the expression of ompG was placed under the control of the pspA promoter [5].
• Negative autoregulation of PspF is continual and not affected by stimuli, like f1 pIV, that induce the pspA to -E operon [3].
• The kinetics of the expression of the pspA gene were determined in an E. coli strain which carried a pspA-lacZ fusion in the chromosome [6].

Biological context of pspA

• Molecular characterization of cog-192 revealed that it is a large chromosomal deletion extending from the 3' end of pspA through to the 5' end of an open reading frame located immediately upstream of ompG [5].

References