Disease relevance of ansA

• L-asparaginase genes in Escherichia coli: isolation of mutants and characterization of the ansA gene and its protein product [1].

High impact information on ansA

• The nucleotide sequence of ansA and flanking regions, comprising 2156 bp, has been determined [2].
• Together, these observations suggest that ansA and ORF1 constitute an operon [2].
• A second open reading frame (ORF1), encoding a protein of Mr 23,336, is found 10 bp downstream from ansA; the ribosome-binding site of ORF1 overlaps the stop codon of ansA [2].
• Strains carrying recombinant plasmids encoding the ansA gene overproduced asparaginase I approximately 130-fold, suggesting that the ansA gene might normally be under negative regulation [1].
• When a DNA fragment containing the ansA gene was used to probe Southern blots of restriction endonuclease-digested E. coli chromosomal DNA, no homologous sequences were revealed other than the expected ansA-containing fragments [1].

Analytical, diagnostic and therapeutic context of ansA

• The ansA gene product has been identified and has a calculated Mr of 35,388; gel filtration of cell extracts indicates that the active form of the enzyme is a dimer [2].

References