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Gene Review:

recX - regulatory protein for RecA

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK2693, JW2668, oraA

Stohl, E.A. et al., Pagès, V. et al., Stohl, E.A. et al., Venkatesh, R. et al., Renzette, N. et al.

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Disease relevance of recX

A gene designated recX (or oraA) is present directly downstream of recA in E. coli; however, the function of RecX is unknown [1].
The recX gene potentiates homologous recombination in Neisseria gonorrhoeae [2].

High impact information on recX

In many eubacteria, coexpression of recX with recA is essential for attenuation of the deleterious effects of recA overexpression; however, the molecular mechanism has remained enigmatic [3].
In vivo, we showed that recX is under control of the LexA repressor and is up-regulated in response to DNA damage [1].
In log-phase cells, dinI mutants have fewer foci than wild type and that recX mutants have more foci than wild type [4].
We were able to complement all these deficiencies by supplying a functional copy of recX elsewhere in the chromosome [2].
The recX loss-of-function mutant showed decreases in pilus phase variation, DNA transformation and DNA repair ability compared with wild type [2].

Biological context of recX

Strains in which the recX gene was disrupted were constructed by insertion of an antibiotic resistance cassette [5].
recX is a small open reading frame located downstream of recA that is conserved in many bacteria [5].

Analytical, diagnostic and therapeutic context of recX

Transcriptional analysis by Northern blots showed that in E. coli the recX gene is SOS-regulated [5].

References

Escherichia coli RecX inhibits RecA recombinase and coprotease activities in vitro and in vivo. Stohl, E.A., Brockman, J.P., Burkle, K.L., Morimatsu, K., Kowalczykowski, S.C., Seifert, H.S. J. Biol. Chem. (2003) [Pubmed]
The recX gene potentiates homologous recombination in Neisseria gonorrhoeae. Stohl, E.A., Seifert, H.S. Mol. Microbiol. (2001) [Pubmed]
RecX protein abrogates ATP hydrolysis and strand exchange promoted by RecA: insights into negative regulation of homologous recombination. Venkatesh, R., Ganesh, N., Guhan, N., Reddy, M.S., Chandrasekhar, T., Muniyappa, K. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
DinI and RecX modulate RecA-DNA structures in Escherichia coli K-12. Renzette, N., Gumlaw, N., Sandler, S.J. Mol. Microbiol. (2007) [Pubmed]
recX, a new SOS gene that is co-transcribed with the recA gene in Escherichia coli. Pagès, V., Koffel-Schwartz, N., Fuchs, R.P. DNA Repair (Amst.) (2003) [Pubmed]

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