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Gene Review:

gltS - glutamate transporter

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3643, JW3628, gltC

Kalman, M. et al., Dougherty, T.J. et al., Deguchi, Y. et al., Fiermonte, G. et al., Kalman, M. et al.

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Disease relevance of gltS

Nucleotide sequence of gltS, the Na+/glutamate symport carrier gene of Escherichia coli B [1].

High impact information on gltS

Identification of the mitochondrial glutamate transporter. Bacterial expression, reconstitution, functional characterization, and tissue distribution of two human isoforms [2].
The nucleotide sequence of the gltS gene coding for an Na+/glutamate symport carrier of Escherichia coli B has been determined, and the amino acid sequence of the carrier protein was deduced [1].
To construct D-glutamate auxotrophs, it is necessary to transfer sequentially the mutated gltS locus, and then the mutated dga locus into the recipient [3].
The second gene, gltS, is located at 82 min. gltS encodes a protein that is involved in the transport of D- and L-glutamic acid into E. coli, and the gltS gene of WM335 was found to contain two missense mutations [3].
A convergent gltS gene is present beyond the spo operon [4].

Biological context of gltS

The gltS null allele gives a Glt- phenotype, i.e. it abolishes the ability of a gltCc host to grow on glutamate as sole carbon and nitrogen source and also confers alpha-methylglutamate resistance [5].

Associations of gltS with chemical compounds

The gltS gene is known to encode a sodium-dependent, glutamate-specific permease [5].
Induction of gltS gene overexpression under control of isopropyl-beta-D-thiogalactoside (IPTG)-inducible promoters severely inhibits growth [5].

References

Nucleotide sequence of gltS, the Na+/glutamate symport carrier gene of Escherichia coli B. Deguchi, Y., Yamato, I., Anraku, Y. J. Biol. Chem. (1990) [Pubmed]
Identification of the mitochondrial glutamate transporter. Bacterial expression, reconstitution, functional characterization, and tissue distribution of two human isoforms. Fiermonte, G., Palmieri, L., Todisco, S., Agrimi, G., Palmieri, F., Walker, J.E. J. Biol. Chem. (2002) [Pubmed]
The Escherichia coli mutant requiring D-glutamic acid is the result of mutations in two distinct genetic loci. Dougherty, T.J., Thanassi, J.A., Pucci, M.J. J. Bacteriol. (1993) [Pubmed]
The nucleotide sequence of recG, the distal spo operon gene in Escherichia coli K-12. Kalman, M., Murphy, H., Cashel, M. Gene (1992) [Pubmed]
Characterization of the Escherichia coli K12 gltS glutamate permease gene. Kalman, M., Gentry, D.R., Cashel, M. Mol. Gen. Genet. (1991) [Pubmed]

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