Disease relevance of rpmH

• The nucleotide sequence of the dnaA gene promoter and of the adjacent rpmH gene, coding for the ribosomal protein L34, of Escherichia coli [1].
• Nucleotide sequence of the Borrelia burgdorferi rpmH gene encoding ribosomal protein L34 [2].
• This protein had previously been characterized only in proteobacteria and gram-positive bacteria. rnpA and the closely linked rpmH gene, which code for the large subunit ribosomal protein L34, have been sequenced [3].

High impact information on rpmH

• The rpmH gene and the dnaA gene are transcribed in the clockwise and counter-clockwise direction, respectively [1].
• This nucleotide sequence contained a coding sequence identical to the amino acid sequence of the ribosomal protein L34 , designated rpmH . The rimA mutation, which affects the maturation of 50S ribosomal particles, may be an allele of the rpmH gene since it maps close to, or within, the L34 coding sequence [1].
• Thus, the organization of the ORFs from ORF44 to ORF638 resembles the organization of genes in the rpmH-gyrB region of the E. coli chromosome [4].

Biological context of rpmH

• The region between the rpmH and dnaA genes contains five promoters that divergently express the ribosomal protein L34 and the proteins of the dnaA operon, including DnaA, the beta clamp of DNA polymerase III holoenzyme, and RecF [5].

References